Renal Disease: Techniques and Protocols by Michael S. GoligorskyRenal Disease: Techniques and Protocols by Michael S. Goligorsky

Renal Disease: Techniques and Protocols

EditorMichael S. Goligorsky

Paperback | November 10, 2010

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Leading experts distill their extensive experience into user-friendly methods for the study of fluid-electrolyte homeostasis and kidney function in health and disease. Described in step-by-step detail, the techniques move from the molecular level to the whole organism, from simple models to integrative physiology. Here the researcher will find established disease models, methods for optimizing renal disease research, as well as much help in choosing imaging techniques for studies of the kidney's structure and function in health, disease, and during embryonic development. Subjects covered include molecular diagnostics, strategies for studying the molecular mechanisms of kidney disease, technical means to assess the functional correlates of disease, and the planning of clinical trials.
Title:Renal Disease: Techniques and ProtocolsFormat:PaperbackDimensions:520 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737380X

ISBN - 13:9781617373800

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Table of Contents

Part I. Optimizing the Usage of Models of Renal DiseaseStandards of Animal Care in Biological ExperimentsEllen M. LeveeModels of Polycystic Kidney DiseasePoornima UpadhyaRat Models of the Metabolic SyndromeStevan P. Tofovic and Edwin K. JacksonModels of GlomerulonephritisRaghu V. Durvasula and Stuart J. ShanklandPart II. Choices of Imaging Techniques in Studies of Renal DiseaseFunctional Studies of the Kidney with Magnetic Resonance ImagingHani B. Marcos, Yantian Zhang, and Peter L. ChoykeUse of Radionuclides to Study Renal FunctionYiyan Liu and M. Donald BlaufoxIntravital VideomicroscopyTokunori Yamamoto and Fumihiko KajiyaConfocal and Two-Photon MicroscopyJános Peti-Peterdi and P. Darwin BellAtomic Force Microscopy in Renal PhysiologyRobert M. HendersonFreeze-Fracture Analysis of Renal-Epithelial Tight JunctionsHiroyuki SasakiPart III. Studies of Renal DevelopmentOrgan Culture of Intact Metanephric KidneysSimon J. M. Welham and Adrian S. WoolfThe Ureteric Bud: Tissue-Culture Approaches to Branching Morphogenesis and Inductive SignalingAlan O. PerantoniStudies of Cell Lineage in the Developing KidneyMaria Luisa S. Sequeira Lopez and R. Ariel GomezTransient Transfection Assays for Analysis of Signal Transduction in Renal CellsRobin L. Maser, Brenda S. Magenheimer, Christopher A. Zien, and James P. CalvetPart IV. Approaches to Study Molecular Mechanisms of DiseaseThe Study of Gene Polymorphisms: How Complex Is Complex Genetic Disease?Sylvia Bähring, Atakan Aydin, and Friedrich C. LuftLaser-Capture MicrodissectionLaura Barisoni and Robert A. StarSerial Analysis of Gene ExpressionM. Ashraf El-Meanawy, Shrinath Barathan, Patrick S. Hayden, Sudha K. Iyengar, Jeffrey R. Schelling, and John R. SedorRNA Labeling and Hybridization of DNA MicroarraysErwin P. Böttinger, Akiva Novetsky, and Jiri ZavadilGene-Expression Analysis of Microdissected Renal BiopsiesClemens D. Cohen and Matthias KretzlerThe Use of SELDI ProteinChip® Array Technology in Renal Disease ResearchEric Fung, Deb Diamond, Anja Hviid Simonsesn, and Scot R. WeinbergerPart V. Technical Means to Assess Functional Correlates of DiseaseClearance Studies in Genetically Altered MiceWilliam T. Noonan and John N. LorenzLong-Term Blood-Pressure Monitoring in Unrestrained AnimalsKaren A. Griffin, Isam Abu-Amarah, and Anil K. BidaniIn Vitro Studies on Renin ReleaseBoye L. Jensen, Ulla G. Friis, and Ole SkøttMitochondrial FunctionJoel M. Weinberg and Pothana SaikumarDetection of Cysteine S-Nitrosylation and Tyrosine 3-Nitration in Kidney ProteinsMark Crabtree, Gang Hao, and Steven S. GrossProducts of Arachidonic Acid MetabolismMairead A. Carroll, John C. McGiff, and Nicholas R. FerreriMethods for Measurements of Heme Oxygenase (HO) Isoforms-Mediated Synthesis of Carbon Monoxide and HO-1 and HO-2 ProteinsNader G. Abraham, Houli Jiang, Michael Balazy, and Alvin I. GoodmanThe Juxtamedullary Nephron PreparationDaniel Casellas and Leon C. MooreTubuloglomerular FeedbackVolker Vallon and Jurgen SchnermannMicrodissected Perfused VesselsThomas L. PalloneRenal Microperfusion TechniquesCharles S. Wingo, I. David Weiner, and Shen-Ling XiaHow to Design a Clinical TrialBryan M. Curtis, Brendan J. Barrett, and Patrick S. ParfreyConclusionMichael S. GoligorskyIndex