RNA Processing: A Practical Approach: Volume I: Rna Processing

Paperback | August 1, 1995

EditorStephen J. Higgins, B. David Hames

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Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of thissubject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end-processing, capping, methylation, splicing, and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all the key stepsrequired for a successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes.RNA processing - A Practical Approach, Volumes I and II, are invaluable laboratory companions for researchers working on mRNA, rRNA, or tRNA gene expression. Their lucid explanatory style and comprehensive coverage of all the key techniques for analysing RNA processing will be welcomed by bothexperienced researchers and

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Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of thissubject. Step-by-step practical protocols fro...

Stephen J. Higgins and B. David Hames are both at the University of Leeds.

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Format:PaperbackDimensions:232 pages, 9.21 × 6.14 × 0.55 inPublished:August 1, 1995Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199633436

ISBN - 13:9780199633432

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Table of Contents

1. Synthesis and purification of RNA substrates2. Characterization of RNA3. Splicing of mRNA precursors in mammalian cells4. Isolation and characterization of ribonucleoprotein complexes5. Analysis of ribonucleoprotein interactions6. Analysis of pre-mRNA splicing in yeastAppendix

Editorial Reviews

"The author's objectives have been well met in these books, which contain very concise and detailed descriptions of current and pertinent protocols for RNA processing....These technical guidebooks would be of benefit for anyone in research who deals with the molecular biology of RNA. The authorshave brought together a fine group of contributors for this book who are highly qualified and authoritative.....Well written by a number of experienced authorities....This set should be in all university and community libraries, and the industrial research field should also find a great deal of usefrom this set as well. It should also be made available to individuals through university bookstores." --Mark A. Jandreski, PhD, Loyola University Medical Center, Doody's Journal"Useful manuals for laboratory technicians looking for practical hints in RNA processing. I highly recommend them as laboratory methods guides for laboratories involved with RNA research and as useful references for those involved in oligonucleotide-based drug development." --BioPharm