RNA-Protein Interactions: A Practical Approach

Paperback | June 1, 1998

EditorChristopher W.J. Smith

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RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in proteinsynthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear andcomprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through tomethods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivoscreening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory stylewith many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field.

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RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in proteinsynthesis, are established as being fundamenta...

Dr Christopher W J Smith, Lecturer in Biochemistry, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Old Addenbrooke's Site, Cambridge CB2 1GA. Tel: 01223 333655 / 333665. Fax: 01223 766002. Email: cwjs1@mole.bio.cam.ac.uk

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Format:PaperbackDimensions:366 pages, 9.21 × 6.14 × 0.79 inPublished:June 1, 1998Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199636508

ISBN - 13:9780199636501

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Table of Contents

1. Applications of chemically synthesized RNA2. Preparation of RNA-Protein complexes for X-ray crystallography and NMR3. Uses of site-specifically modified RNAs constructed by RNA ligation4. The Electrophoretic Mobility Shift Assay for RNA Binding Proteins5. Affinity methods for isolating RNA-binding proteins6. Synthetic lethal/enhancer screening to identify snRNA-protein and protein-protein interactions in yeast pre-mRNA splicing7. Detecting RNA-Protein Interactions and Isolating cDNA Clones by Northwestern Screening8. A three-hybrid system to detect and analyse RNA-protein interactions in vivo9. In vivo selection of specific RNA-binding polypeptides using a transcription anti-termination reporter assay10. Foot-printing and modification-interference analysis of binding sites on RNA11. Analysis of RNA-Protein and RNA-RNA contacts between ribosomes and functional ligands using site-directed cross-linking techniques12. In vitro selection of nucleic acid ligands

Editorial Reviews

"There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitrotranscription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimentalprotocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover."--TheQuarterly Review of Biology