RNA Viruses: A Practical Approach by Alan CannRNA Viruses: A Practical Approach by Alan Cann

RNA Viruses: A Practical Approach

EditorAlan Cann

Paperback | May 15, 2000

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RNA Viruses: A Practical Approach is wide ranging in scope, from emerging technology such as reverse genetics and retrovirus vectors, to money saving tips - how to make your own silica particles for high efficiency RNA extraction and liposomes for cell transfection! Chapter one covers thefundamentals of investigating RNA virus genome structure at a molecular level. Chapters two and three describe techniques for mutagenesis of RNA genomes and analysis of transcription. Chapter four deals with RNA virus-encoded proteinases, an important aspect of the control of RNA virus geneexpression. Chapter five considers retrovirus oncogenesis and chapter six analysis of RNA virus quasispecies. Chapter seven describes systems for investigation of in vitro replication of positive-stranded viruses and chapter eight the packaging of RNA virus genomes. In addition to the technicalaspects of reverse genetics and retrovirus vectors, both of the final two chapters also consider ethical aspects of these new technologies.
Alan Cann is at University of Leicester.
Title:RNA Viruses: A Practical ApproachFormat:PaperbackPublished:May 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199637164

ISBN - 13:9780199637164

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Table of Contents

Investigation of RNA Virus Genome StructureMutagenesis of RNA Virus GenomesAnalysis of Transcriptional Control in RNA Virus InfectionsAnalysis of RNA Virus-encoded ProteinasesDetection and Analysis of Host Gene Targets for Oncogenic RetrovirusesAnalysis of RNA Virus QuasispeciesIn Vitro Replication of RNA VirusesPackaging of Segmented and non-segmented RNA Virus GenomesRNA Virus Reverse GeneticsDevelopment of RNA Virus Vectors for Gene Delivery