Selection Of The Hplc Method In Chemical Analysis

Paperback | November 14, 2016

bySerban C. Moldoveanu, Victor David

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Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high performance liquid chromatography, providing exacting criteria for method selection, development, and validation. High performance liquid chromatography is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection. This book, for the first time, presents a systematic approach for sorting through the available information, also providing a critical analysis of the progress in HPLC for selecting a specific analysis. It is an inclusive, go-to reference for HPLC method selection, development, and validation. Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results Leads researchers to the best choice of an HPLC method from the overabundance of information existing in the field Provides exacting criteria for HPLC method selection, development, and validation Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field

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From the Publisher

Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high performance liquid chromatography, providing exacting criteria for method selection, development, and validation. High performance liquid chromatography is the most common analytical technique currently practiced in chemistry. However, the pr...

Dr. Serban Moldoveanu is Sr. Principal Scientist at R. J. Reynolds Tobacco Co. His research activity is focused on various aspects of chromatography including method development for the analysis by GC/MS, HPLC, and LC/MS/MS of natural products and cigarette smoke. He also performed research on pyrolysis of a variety of polymers and sma...

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Format:PaperbackDimensions:598 pages, 8.75 × 6.35 × 0.68 inPublished:November 14, 2016Publisher:Elsevier Science & TechLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128036842

ISBN - 13:9780128036846

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Table of Contents

1. Start of the implementation of a new HPLC method 2. Short overviews of analytical techniques not containing an independent separation step 3. Short overviews of main analytical techniques containing a separation step 4. Basic information regarding the HPLC techniques 5. Properties of analytes and matrices determining HPLC selection 6. General aspects regarding the HPLC analytical column 7. RP-HPLC analytical columns 8. Polar analytical columns 9. Stationary phases and columns for ion exchange, ion-moderated, and ligand exchange chromatography 10. Stationary phases and columns for chiral chromatography 11. Stationary phases and columns for size exclusion 12. Stationary phases and columns for immunoaffinity type separations 13. Solvents, buffers, and additives used in the mobile phase 14. Gradient elution 15. The Practice of HPLC

Appendix 1. USP classification of HPLC columns Appendix 2. Hydrophobic stationary phases Appendix 3. HILIC and NPC stationary phases Appendix 4. Ion exchange and ion moderated stationary phases Appendix 5. Chiral stationary phases Appendix 6. Size exclusion stationary phases Appendix 7. Properties of mobile phase components