Septic Shock Methods and Protocols by Thomas J. EvansSeptic Shock Methods and Protocols by Thomas J. Evans

Septic Shock Methods and Protocols

EditorThomas J. Evans

Paperback | November 9, 2010

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An interdisciplinary panel of expert investigators describe proven methods for investigating the often deadly mechanisms of septic shock. Drawing on insights from a wide variety of fields-ranging from molecular and cellular biology to the physiological measurement of entire animals-these experienced research workers detail the most important techniques for studying sepsis involving endotoxins, toxins derived from Gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and primary cell cultures. They also treat a number of significant techniques that are not well described in the literature, including the the Limulus endotoxin assay, the bioassay of TNF, the purification of bacterial endotoxin and superantigens, and the culture of primary human cells. Multidisciplinary and state-of-the-art, Septic Shock Methods and Protocols offers both experimental and clinical investigators a gold-standard collection of key methods for illuminating the basic pathophysiological processes that occur following infection.
Title:Septic Shock Methods and ProtocolsFormat:PaperbackDimensions:224 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371548

ISBN - 13:9781617371547

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Table of Contents

Part I. Endotoxin. Assay of Endotoxin by Limulus Amebocyte Lysate, Paul A. Ketchum and Thomas J. Novitsky. Preparation of Endotoxin from Pathogenic Gram-Negative Bacteria, Alexander Shnyra, Michael Luchi, and David C. Morrison. Assay of Anti-Endotoxin Antibodies, Lore Brade. Isolation of the Bactericidal/Permeability-Increasing Protein from Polymorphonuclear Leukocytes by Reversible Binding to Target Bacteria, Jerrold Weiss. Purification of Lipopolysaccharide Binding Protein, Didier Heumann. Part II. Other Bacterial Products. Purification of Streptococcal Pyrogenic Exotoxin A, Manuela Roggiani and Patrick M. Schlievert. Assay for Superantigens, Shiranee Sriskandan. Part III. Cytokines. Bioassay for Tumor Necrosis Factors-a and b, Thomas J. Evans. Assay of Soluble Tumor Necrosis Factor Receptors, Maarten G. Bouma and Wim A. Buurman. Whole-Blood Assays for Cytokine Production, Daniel G. Remick, David E. Newcomb, and Jon S. Friedland. Part IV. Nitric Oxide and Other Reactive Nitrogen Intermediates. Nitric Oxide Synthase Inhibitors, Dilani K. Siriwardena, Hajime Tagori, Christoph Thiemermann. In Situ Detection of Nitric Oxide, Tadeusz Malinski. Immunochemical Detection of Nitric Oxide Synthase in Human Tissue, Lee D. K. Buttery and Julia M. Polak. Anti-Nitrotyrosine Antibodies for Immunohistochemistry, Liliana Viera, Yao Zu Ye, and Joseph S. Beckman. Detection of Peroxynitrite in Biological Fluids, Stuart Malcolm, Raymond Foust III, Caryn Hertkorn, and Harry Ischiropoulos. Part V. Cell Culture Techniques. Myocardial Cells in Culture to Study Effects of Cytokines, Kevin A. Krown and Roger A. Sabbadini. Culture of Primary Human Bronchial Epithelial Cells, Joanna Picot. Primary Culture of Human Proximal Renal Tubular Epithelial Cells, Paul A. Glynne. Index

Editorial Reviews

"this text is a fine laboratory reference. . . the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators and students engaged in septic shock/infection studies. "-Doody's Health Sciences Book Review Journal