Septic Shock Methods and Protocols by Thomas J. EvansSeptic Shock Methods and Protocols by Thomas J. Evans

Septic Shock Methods and Protocols

byThomas J. Evans

Paperback | November 9, 2010

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Septic shock remains a serious medical condition with high mortality. Despite many advances in intensive care medicine and antibiotic devel- ment, this has not changed appreciably in the last 20 years. Frustratingly, over the same period of time, enormous advances have been made in understa- ing the underlying pathogenic mechanisms of this condition. This has resulted in the development of several novel therapies for septic shock, which, despite excellent theoretical grounds for their efficacy, have failed in altering mort- ity attributable to sepsis. The reasons for these failures are multiple, but it is clear that further research is required aimed at increasing our understanding of the basic pat- physiological processes that occur following infection. Research into septic shock draws upon a number of different disciplines, ranging from molecular and cellular biology to physiological measurements on whole animals. Septic Shock Methods and Protocols is an attempt to draw together into one volume a number of protocols that are of use in the investigation of the mechanisms of septic shock. I have divided the book into five sections. The first deals with endotoxin, the lipopolysaccharide component of the Gram-negative cell m- brane that can mimic many of the features of septic shock. Gram-positive organisms are found increasingly as causes of septic shock, and several - pects of toxins produced from these bacteria are considered in the second s- tion.
Title:Septic Shock Methods and ProtocolsFormat:PaperbackDimensions:208 pagesPublished:November 9, 2010Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371548

ISBN - 13:9781617371547

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Table of Contents

Part I. Endotoxin. Assay of Endotoxin by Limulus Amebocyte Lysate, Paul A. Ketchum and Thomas J. Novitsky. Preparation of Endotoxin from Pathogenic Gram-Negative Bacteria, Alexander Shnyra, Michael Luchi, and David C. Morrison. Assay of Anti-Endotoxin Antibodies, Lore Brade. Isolation of the Bactericidal/Permeability-Increasing Protein from Polymorphonuclear Leukocytes by Reversible Binding to Target Bacteria, Jerrold Weiss. Purification of Lipopolysaccharide Binding Protein, Didier Heumann. Part II. Other Bacterial Products. Purification of Streptococcal Pyrogenic Exotoxin A, Manuela Roggiani and Patrick M. Schlievert. Assay for Superantigens, Shiranee Sriskandan. Part III. Cytokines. Bioassay for Tumor Necrosis Factors-a and b, Thomas J. Evans. Assay of Soluble Tumor Necrosis Factor Receptors, Maarten G. Bouma and Wim A. Buurman. Whole-Blood Assays for Cytokine Production, Daniel G. Remick, David E. Newcomb, and Jon S. Friedland. Part IV. Nitric Oxide and Other Reactive Nitrogen Intermediates. Nitric Oxide Synthase Inhibitors, Dilani K. Siriwardena, Hajime Tagori, Christoph Thiemermann. In Situ Detection of Nitric Oxide, Tadeusz Malinski. Immunochemical Detection of Nitric Oxide Synthase in Human Tissue, Lee D. K. Buttery and Julia M. Polak. Anti-Nitrotyrosine Antibodies for Immunohistochemistry, Liliana Viera, Yao Zu Ye, and Joseph S. Beckman. Detection of Peroxynitrite in Biological Fluids, Stuart Malcolm, Raymond Foust III, Caryn Hertkorn, and Harry Ischiropoulos. Part V. Cell Culture Techniques. Myocardial Cells in Culture to Study Effects of Cytokines, Kevin A. Krown and Roger A. Sabbadini. Culture of Primary Human Bronchial Epithelial Cells, Joanna Picot. Primary Culture of Human Proximal Renal Tubular Epithelial Cells, Paul A. Glynne. Index

Editorial Reviews

"this text is a fine laboratory reference. . . the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators and students engaged in septic shock/infection studies. "-Doody's Health Sciences Book Review Journal