Signalling by Inositides: A Practical Approach

Paperback | June 1, 1997

EditorStephen Shears

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The metabolism and functions of inositol phosphates impinge on various branches of biochemistry, physiology, and molecular biology, and methodological information is in consequence scattered far and wide. This book unites a selection of the most fundamental and commonly used techniques fromleading international signal transduction laboratories, and brings together many valuable protocols for purifying and assaying inositides and related compounds. A novel feature is a catalogue of non-commercial sources of synthetic inositide analogues.

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From the Publisher

The metabolism and functions of inositol phosphates impinge on various branches of biochemistry, physiology, and molecular biology, and methodological information is in consequence scattered far and wide. This book unites a selection of the most fundamental and commonly used techniques fromleading international signal transduction labo...

Dr Stephen B. Shears, Inositol Lipid Section, Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, PO Box 12233 Research Triangle Park, NC 27709 USA. Tel: 001 919 541 0793. Fax: 001 919 541 0559. Email: shears@niehs.nih.gov
Format:PaperbackDimensions:250 pages, 9.21 × 6.14 × 0.59 inPublished:June 1, 1997Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199636389

ISBN - 13:9780199636389

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Table of Contents

1. Assaying inositol phospholipid turnover in plant cells2. Measurement of inositol phosphate turnover in intact cells and cell-free systems3. Mass assay of inositol and its use to assay inositol polyphosphates4. Phospholipase C-gamma 15. Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-beta isoenzymes6. Purification and assay of phospholipase C-delta7. The purification and assay of inositol polyphosphate-binding proteins8. Mass assay of inositol 1,4,5-trisphosphate and phosphatidylinositol 4,5-bisphosphate9. Desalting inositol polyphosphates by dialysis10. The Ins(1,4,5)P3 receptor11. Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase12. Phosphoinositide 3-kinase

Editorial Reviews

"The graphs, diagrams, tables, and few half-tone illustrations are of good to excellent quality...The book is small and easy to handle and read...Complete list of suppliers at the end of the book....This small, well-written book should be useful for those who do not regularly work in this field. Itcontains clearly written and presented lab protocols that should be easy for anyone to follow and make work in their own laboratory."--Doody's Journal