Single Domain Antibodies: Methods and Protocols by Dirk SaerensSingle Domain Antibodies: Methods and Protocols by Dirk Saerens

Single Domain Antibodies: Methods and Protocols

EditorDirk Saerens, Serge Muyldermans

Hardcover | August 11, 2012

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The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Title:Single Domain Antibodies: Methods and ProtocolsFormat:HardcoverDimensions:585 pages, 10 × 7.01 × 0.01 inPublished:August 11, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161779967X

ISBN - 13:9781617799679

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Table of Contents

Part I: Overview of Single Domain Antibodies1. From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small            Jean-Luc Teillaud2. Introduction to Heavy Chain Antibodies and Derived Nanobodies            Cécile Vincke and Serge Muyldermans3. Overview and Discovery of IgNARs and Generation of VNARs             Stewart D. NuttallPart II: Single Domain Antibody Library Construction4. Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and V? Domain Antibodies             Olga Ignatovich, Laurent Jespers, Ian M, Tomlinson, and Ruud M.T. de Wildt5. Preparation of a Naïve Library of Camelid Single Domain Antibodies             Aurelien Olichon and Ario de MarcoPart III: Selection of Single Domain Antibodies6. Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation             Peter Verheesen and Toon Laeremans7. Semi-Automated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens             Jyothi Kumaran, Roger MacKenzie, and Mehdi Arbabi-Ghahroudi8. Pichia Surface Display: A Tool for Screening Single Domain Antibodies             Kristof De Schutter and Nico Callewaert9. Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method             Mireille Pellis, Serge Muyldermans, and Cécile Vincke10. Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies             Tomoyuki Tanaka and Terence H. Rabbitts 11. Selection of Functional Single Domain Antibody Fragments for Interfering with Protein-Protein Interactions Inside Cells: A "One Plasmid" Mammalian Two-Hybrid System             Tomoyuki Tanaka and Terence H. Rabbitts12. Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization             Armin Sepp and Andrew Griffiths13. Selection of VHHs Under Application Conditions             Edward Dolk, Theo Verrips, and Hans de Haard14. Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies             Greg Hussack, Mehdi Arbabi-Ghahroudi, C. Roger MacKenzie, and Jamshid Tanha15. Selection of VHH Antibody Fragments that Recognize Different A? Depositions Using Complex Immune Libraries             Rinse Klooster, Kim S. Rutger, and Sylvère van der MaarelPart IV: Expression of Single Domain Antibodies and Derivatives16. Expression of Single-Domain Antibodies in Bacterial Systems             Toya Nath Baral and Mehdi Arbabi-Ghahroudi17. Expression of VHHs in S. cerevisiae             Andrea Gorlani, Hans de Haard, and Theo Verrips18. Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells             Vishal Agrawal, Igor Slivac, Sylvie Perret, Louis Bisson, Gilles St-Laurent, Yanal Murad, Jianbing Zhang, and Yves Durocher19. Production of Camel-Like Antibodies in Plants             Sylvie De Buck, Vikram Virdi, Thomas De Meyer, Kirsten De Wilde, Robin Piron, Jonah Nolf, Els Van Lerberge, Annelies De Paepe, and Ann DepickerPart V: Improvement and Applications of Single Domain Antibodies20. Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains             Raffi Tonikian and Sachdev S. Sidhu21. Solubility and Stability Engineering of Human VH Domains             Dae Young Kim, Wen Ding, and Jamshid Tanha22. Improvement of Proteolytic Stability through In Silico Engineering             Lucy Rutten, Hans de Haard, and Theo Verrips23. Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display             Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ24. Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction             Yoshihisa Hagihara and Dirk Saerens25. Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond             Greg Hussack, C. Roger MacKenzie, and Jamshid Tanha26. Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display             Akiko Koide and Shohei Koide27. Multivalent Display of Single-Domain Antibodies             Jianbing Zhang and C. Roger MacKenzie28. Methods for Determining the PK Parameters of AlbudAbs and of Long Serum Half Life Drugs Made Using the AlbudAb Technology             Daniel Rycroft and Lucy J. Holt29. Fluorescent Protein Specific Nanotraps to Study Protein-Protein Interactions and Histone-Tail Peptide Binding             Garwin Pichler, Heinrich Leonhardt, and Ulrich Rothbauer30. Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide             Catarina Xavier, Nick Devoogdt, Sophie Hernot, Ilse Vaneycken, Matthias D'Huyvetter, Jens De Vos, Sam Massa, Tony Lahoutte, and Vicky Caveliers31. Nanobody-Based Chromatin Immunoprecipitation             Trong Nguyen Duc, Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, Eveline Peeters, Daniel Charlier, and Serge Muyldermans32. User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags             Ario de Marco33. Application of Single Domain Antibodies in Tumor Histochemistry             Kien T. Mai and C. Roger MacKenziePart VI: Case Studies 34. Nanobodies as Structural Probes of Protein Misfolding and Fibril Formation             Erwin De Genst and Christopher M. Dobson35. Molecular Imaging Using Nanobodies: A Case Study             Nick Devoogdt, Catarina Xavier, Sophie Hernot, Ilse Vaneycken, Matthias D'Huyvetter, Jens De Vos, Sam Massa, Patrick De Baetselier, Vicky Caveliers, and Tony Lahoutte36. Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis             Kourosh Zolghadr, Jacqueline Gregor, Heinrich Leonhardt, and Ulrich Rothbauer            Stewart D. Nuttall