Single-molecule Enzymology: Nanomechanical Manipulation And Hybrid Methods

Hardcover | January 10, 2017

byMaria SpiesEditorYann R Chemla

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Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion

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Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluo...

Associate Professor of Biochemistry, Carver College of Medicine, University of Iowa, USAAssociate Professor of Physics and Biophysics, Department of Physics, University of Illinois at Urbana-Champaign, USA

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Format:HardcoverDimensions:412 pages, 9.41 × 7.24 × 0.98 inPublished:January 10, 2017Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128093102

ISBN - 13:9780128093108

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Table of Contents

  1. Single-molecule optical trapping techniques to study molecular mechanisms of a replisome
    Michelle D. Wang
  2. How to measure load-dependent kinetics of individual motor molecules without a force clamp
    Henrik Flyvbjerg, James Spudich, Kim Mortensen and Jongmin Sung
  3. Studying the mechanochemistry of processive cytoskeletal motors with an optical trap
    Ahmet Yildiz
  4. Recent advances in biological single-molecule applications of optical tweezers and fluorescence microscopy
    Erwin Peterman, Gijs Wuite and Maryam Hashemi Shabestari
  5. Combining single-molecule technologies: DNA tightropes and optical trapping of complex micro-structures
    Neil Kad and Jingyu Wang
  6. Direct Visualization of Helicase Dynamics using Fluorescence Localization and Optical Trapping
    Taekjip Ha
  7. High-resolution optical tweezers combined with single-molecule confocal microscopy
    Yann R. Chemla
  8. Single-stranded DNA curtains for studying homologous recombination
    Eric C. Greene
  9. Inserting extrahelical structures into long DNA substrates for single-molecule studies of DNA mismatch repair
    Ilya J. Finkelstein
  10. Single-molecule insight into target recognition by CRISPR-Cas complexes
    Ralf Seidel
  11. Single-molecule enzymology
    H. Peter Lu
  12. MT - helicases/replication
    Vincent Croquette
  13. Preparation of DNA substrates and functionalized glass surfaces for correlative nanomanipulation and colocalization (NanoCOSM) of single molecules
    Terence Strick
  14. Measuring force induced dissociation kinetics of protein complexes using single molecule atomic force microscopy
    Sanjeevi Sivasankar
  15. Improved force spectroscopy using focused-ion-beam modified cantilevers
    Thomas T. Perkins and Jaevyn K. Faulk
  16. Single-molecule characterization of DNA-protein interactions using nanopore biosensors
    Amit Meller
  17. Subangstrom measurements of enzyme function using a biological nanopore, SPRNT
    Jens H. Gundlach
  18. Multiplexed, tethered particle microscopy for studies of DNA-enzyme dynamics
    Laura Finzi