Spectrophotometry and Spectrofluorimetry: A Practical Approach

Paperback | March 15, 2000

EditorMike Gore

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Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operationsof most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolvedfluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that havetraditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this methodof utilizing the vast arrays of experimental data provided by current instrumentation.

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From the Publisher

Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operationsof most laboratories in the chemical, biolog...

Mike Gore is at University of Southampton.
Format:PaperbackPublished:March 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638128

ISBN - 13:9780199638123

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Table of Contents

Introduction to light absorption; visible and ultraviolet spectraFluorescence principles and measurementTime resolved fluorescence and phosphorescence spectroscopyAn introduction to circular dichroismQuantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methodsSteady state kineticsSpectrophotometric assaysStopped flow spectroscopyStopped flow fluorescenceStopped flow circular dichroismSpectrophotometry and fluorimetry of cellular compartments and intracellular processesUse of optical spectroscopic methods to study the thermodynamic stability of proteinsThe use of spectroscopic techniques in the study of protein stability

Editorial Reviews

'...Practical advice is given by experts in the different topics, together with lists of useful literature'Librairie