Strain Engineering: Methods and Protocols by James A. WilliamsStrain Engineering: Methods and Protocols by James A. Williams

Strain Engineering: Methods and Protocols

EditorJames A. Williams

Hardcover | July 15, 2011

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Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular BiologyT series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.

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Title:Strain Engineering: Methods and ProtocolsFormat:HardcoverDimensions:491 pages, 10 × 7.01 × 0.1 inPublished:July 15, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617791962

ISBN - 13:9781617791963

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Table of Contents

Part 1. E. coli  1. Bacterial Genome Reengineering            Jindan Zhou and Kenneth E. Rudd 2. Targeted Chromosomal Gene Knockout using PCR Fragments            Kenan C. Murphy 3. Scarless Chromosomal Gene Knockout Methods            Bong Hyun Sung, Jun Hyoung Lee, and Sun Chang Kim 4. Random Chromosomal Gene Disruption In vivo using Transposomes            Les M. Hoffman 5. Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection            Elie J. Diner, Fernando Garza-Sánchez, and Christopher S. Hayes 6. Microarray-based Genetic Footprinting Strategy to Identify Strain Improvement Genes after Competitive Selection of Transposon Libraries            Alison K. Hottes and Saeed Tavazoie 7. Optimization of Synthetic Operons using Libraries of Post-Transciptional Regulatory Elements            Daniel E. Agnew and Brian F. Pfleger 8. Marker Free Chromosomal Expression of Foreign and Native Genes in E. coli            Chung-Jen Chiang, Po Ting Chen, Shan-Yu Chen, and Yun-Peng Chao 9. Array-based Synthetic Genetic Screens to Map Bacterial Pathways and Functional Networks in Escherichia coli            Mohan Babu, Alla Gagarinova, and Andrew Emili 10. Assembling New E. coli Strains by Transduction using Phage P1             Sean D. Moore Part II. Saccharomyces cerevisiae 11. Yeast Bioinformatics and Strain Engineering Resources            Audrey L. Atkin 12. Delete and Repeat: A Comprehensive Toolkit for Sequential Gene Knock-out in the Budding Yeast Saccharomyces cerevisiae            Johannes H. Hegemann and Sven Boris Heick 13. Genome-wide Transposon Mutagenesis in Saccharomyces cerevisiae and Candida albicans            Tao Xu, Nikë Bharucha, and Anuj Kumar  14. Signature-tagged Mutagenesis to Characterize Genes Through Competitive Selection of Barcoded Genome Libraries            Julia Oh and Corey Nislow   15. Global Strain Engineering by Mutant Transcription Factors            Amanda M. Lanza and Hal S. Alper 16. Genomic Promoter Replacement Cassettes to Alter Gene Expression in the Yeast Saccharomyces cerevisiae            Andreas Kaufmann and Michael Knop   Part III. Strain Engineering Other Industrially Important Microbes 17. Microbial Genome Analysis and Comparisons: Web-based Protocols and Resources            Medha Bhagwat and Arvind A. Bhagwat 18. Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria            Tohru Suzuki and Kazumasa Yasui 19. Broad-host-range Plasmid Vectors for Gene Expression in Bacteria            Rahmi Lale, Trygve Brautaset, and Svein  Valla 20. Simple Method for Introducing Marker-free Deletions in the Bacillus subtilis Genome            Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, and Naotake Ogasawara  21. Transposon-mediated Random Mutagenesis of Bacillus subtilis            Adam C. Wilson and Hendrik Szurmant  22. Integrative Food Grade Expression System for Lactic Acid Bacteria            Grace L. Douglas, Yong Jun Goh and Todd R. Klaenhammer  23. ClosTron-mediated Engineering of Clostridium            Sarah A. Kuehne, John T.  Heap, Clare M. Cooksley, Stephen T. Cartman, and Nigel P. Minton 24. High Throughput Transposon Mutagenesis of Corynebacterium glutamicum            Nobuaki Suzuki , Masayuki Inui, and Hideaki Yukawa  25. Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis            Katherine M. Pappas  26. Engineering Thermoacidophilic Archaea using Linear DNA Recombination            Yukari Maezato, Karl Dana, and Paul Blum  27. Targeted Gene Disruption in Koji Mold Aspergillus oryzae            Jun-ichi Maruyama and Katsuhiko Kitamoto  28. Selectable and Inheritable Gene Silencing through RNA Interference in the Unicellular Alga Chlamydomonas reinhardtii            Karin Van Dijk and Nandita Sarkar