Subcellular Fractionation: A Practical Approach

Paperback | February 1, 1997

EditorJ. M. Graham, D. Rickwood

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Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (theisolation and fractionation chapters), to characterization of the separated organelles.

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Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (theisolation and fractionation chapters), to chara...

John Graham is at Merseyside Innovation Centre, Liverpool. David Rickwood is at University of Essex.

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Format:PaperbackDimensions:360 pages, 9.21 × 6.14 × 0.79 inPublished:February 1, 1997Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199634947

ISBN - 13:9780199634941

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Table of Contents

1. Homogenization of tissues and cells2. Isolation of subcellular fragments3. Isolation and characterization of nuclei and nuclear subfractions4. Subcellular fractionation of mitochondria5. Isolation and characterization of peroxisomes6. Lysosomes and endocytosis7. The membranes of the secretory and exocytic pathways8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells9. Ribosomes and polysomes10. Electron microscopy of organelles