Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols by Peter M. RabinovichSynthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols by Peter M. Rabinovich

Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols

EditorPeter M. Rabinovich

Hardcover | January 8, 2013

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Synthetic mRNA is an attractive tool for mammalian cell reprogramming that  can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing  the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery.  Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.
Title:Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and ProtocolsFormat:HardcoverDimensions:335 pages, 10 × 7.01 × 0.01 inPublished:January 8, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627032592

ISBN - 13:9781627032599

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Table of Contents

Part I. mRNA Synthesis, Purification, and Transfer 1. Cell Engineering with Synthetic Messenger RNA              Peter M. Rabinovich and Sherman M. Weissman 2. In vitro Transcription of Long RNA Containing Modified Nucleosides Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karikó 3. HPLC Purification of in vitro Transcribed Long RNA             Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karikó 4. Synthetic mRNAs with Superior Translation and Stability Properties            Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads 5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA              James G. Hecker  6. Light-Induced mRNA Transfection               Sigurd Leinæs Bøe and Eivind Hovig 7. Engineering B Cells with mRNA              Jaewoo Lee, David Boczkowski, and Smita Nair 8. Programming Human Dendritic Cells with mRNA             Jaewoo Lee, David Boczkowski, and Smita Nair 9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process            Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa 10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation             Melanie Galla, Axel Schambach, and Christopher Baum 11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells            Frank Breinig, Tanja Breinig, and Manfred J. Schmitt  Part 2. Modulation and monitoring of cell metabolism        12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy            Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken 13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors             Noriko Shimasaki and Dario Campana 14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA            Peizhe Wang and Jie Na 15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies            Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin 16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA            Chantal Pichon and Patrick Midoux 17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA             Özcan Met and Inge Marie Svane 18. mRNA electroporation as a Tool for Immunomonitoring            Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman 19. mRNA PCR-Based Epitope Chase Method                       Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe  Part 2. Modulation and monitoring of cell metabolism        12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy            Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken 13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors             Noriko Shimasaki and Dario Campana 14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA            Peizhe Wang and Jie Na 15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies            Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin 16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA            Chantal Pichon and Patrick Midoux 17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA             Özcan Met and Inge Marie Svane 18. mRNA electroporation as a Tool for Immunomonitoring            Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman 19. mRNA PCR-Based Epitope Chase Method                       Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe