Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols by Danny M. HattersTandem Repeats in Genes, Proteins, and Disease: Methods and Protocols by Danny M. Hatters

Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols

EditorDanny M. Hatters, Anthony J. Hannan

Hardcover | May 30, 2013

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The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences.  Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine.  Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states.  This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular  and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.
Title:Tandem Repeats in Genes, Proteins, and Disease: Methods and ProtocolsFormat:HardcoverDimensions:269 pages, 10 × 7.01 × 0.98 inPublished:May 30, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627034374

ISBN - 13:9781627034371

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Table of Contents

1. Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded ProteinsAndrey S. Tsvetkov, D. Michael Ando & Steven Finkbeiner 2. Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on SurfacesKathleen A. Burke and Justin Legleiter 3. Morphometric Analysis of Huntington's Disease Neurodegeneration in DrosophilaWan Song, Marianne R. Smith, Adeela Syed, Tamas Lukacsovich, Brett A. Barbaro, Judith Purcell, Doug J. Bornemann, John Burke and J. Lawrence Marsh 4. Size Analysis of Polyglutamine Protein Aggregates using Fluorescence Detection in an Analytical UltracentrifugeSaskia Polling, Danny M. Hatters, and Yee-Foong Mok 5. A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant ProteinsAmy L. Robertson and Stephen P. Bottomley 6. Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine AggregationYasmin M. Ramdzan, Rebecca Wood, Danny M. Hatters 7. Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain DisordersEmma L. Burrows and Anthony J. Hannan 8. PCR Amplification and Sequence Analysis of GC-rich Sequences: Aristaless-Related Homeobox ExampleTan M.H., Gécz J., Shoubridge C. 9. Challenges of "sticky" Co-Immunoprecipitation; Polyalanine Tract Protein-Protein InteractionsMattiske T.R., Tan M.H., Gécz J. and Shoubridge C. 10. Molecular Pathology of Polyalanine Expansion Disorders-new Perspectives from Mouse ModelsJames N Hughes and Paul Q Thomas 11. Yeast as a Platform to Explore Polyglutamine Toxicity and AggregationMartin L. Duennwald 12. Immunobased Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological SamplesGregor P. Lotz and Andreas Weiss  13. Modeling and Analysis of Repeat RNA Toxicity in DrosophilaSamaraweera, S.E. , O'Keefe, L.V. , van Eyk, C.L. , Lawlor, K.T., Humphreys D.T., Suter, C.M. and Richards, R.I. 14. Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel ElectrophoresisMats Holmberg, Ellen A.A. Nollen 15. Kinetic Analysis of Aggregation Data Regina M. Murphy 16. A Bioinformatics Method for Identifying Q/N-rich Prion-like Domains in ProteinsEric D. Ross, Kyle S. MacLea, Charles Anderson, and Asa Ben-Hur 17. Detecting Soluble PolyQ Oligomers and Investigating their Impact on Living Cells using Split-GFPPatrick Lajoie and Erik Lee Snapp 18. Cell Biological Approaches To Investigate Polyglutamine-Expanded AR MetabolismLori J. Cooper and Diane E. Merry