Tetrahymena Thermophila

Other | September 1, 1999

byWilson, Leslie, Leslie Wilson

not yet rated|write a review
Tetrahymena thermophilais emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations withT. thermophilahave yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for usingTetrahymenain the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning toTetrahymenaas the experimental system of choice.

Key Features
* Contains both theoretical and practical issues in 30 chapters contributed by the world authorities onTetrahymena
* Indispensible for both the novice and the experienced researcher
* Overviews the history, cell biology, and genetics of the organism
* Describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope
* Illustrates how the methods can be applied to solve various cell biological problems
* Reviews recently developed strategies for altering gene expression

Pricing and Purchase Info

$176.89 online
$229.66 list price (save 22%)
In stock online
Ships free on orders over $25

From the Publisher

Tetrahymena thermophilais emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations withT. thermophilahave...

James Forney received a Ph.D. in Molecular Biology at Indiana University. In 1988, after postdoctoral work at the University of California, Berkeley, he joined the faculty at Purdue University where he is now Professor of Biochemistry. Throughout his career, James Forney has maintained an interest in gene expression and DNA rearrangeme...

other books by Wilson, Leslie

Slavery of Faith: The untold story of the Peoples Temple from the eyes of a thirteen year old, her…
Slavery of Faith: The untold story of the Peoples Templ...

Paperback|Jan 9 2009

$22.23 online$23.50list price(save 5%)
The Last Train From Kummersdorf
The Last Train From Kummersdorf

Paperback|Jun 30 2015

$12.99

Americana: Readings in Popular Culture, Revised Edition
Americana: Readings in Popular Culture, Revised Edition

Kobo ebook|Apr 1 2010

$20.19 online$26.18list price(save 22%)
see all books by Wilson, Leslie
Format:OtherDimensions:588 pages, 1 × 1 × 1 inPublished:September 1, 1999Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080524958

ISBN - 13:9780080524955

Customer Reviews of Tetrahymena Thermophila

Reviews

Extra Content

Table of Contents

Overview:
D.L. Nanney and E.M. Simon, Laboratory and Evolutionary History ofTetrahymena thermophila.
J. Frankel, Cell Biology ofTetrahymena thermophila.
K.M. Karrer,TetrahymenaGenetics: Two Nuclei are Better Than One.
Methods:
E. Orias, E.P. Hamilton, and J.D. Orias,Tetrahymenaas a Laboratory Organism: Useful Strains, Cell Culture, and Cell Line Maintenance.
P.J. Bruns, H.R. Smith, and D. Cassidy-Hanley, Long-term Storage.
E.P. Hamilton and E. Orias, Genetic Crosses: Setting up Crosses, Testing Progeny, and Isolating Phenotypic Assortants.
P.J. Bruns and D. Cassidy-Hanley, Methods for Genetic Analysis.
S.L. Allen, Isolation of Micronuclear and Macronuclear DNA.
E. Orias and E.P. Hamilton, Genetically Sorting a Collection ofTetrahymenaMutants.
E.P. Hamilton and E. Orias, Genetically Mapping New Mutants and Cloned Genes.
D.G. Pennock, Selection of Motility Mutants.
K.R. Stuart and E.S. Cole, Nuclear and Cytoskeletal Fluorescence Microscopy Techniques.
E.S. Cole and K.R. Stuart, Nuclear and Cortical Histology for Brightfield Microscopy.
W. Dentler, Fixation ofTetrahymenaCells for Electron Microscopy.
R.H. Gavin, J.G. Hoey, and J.A. Garcés, Immunoelectron Microscopy ofTetrahymena.
Cell Biology:
A.P. Turkewitz, N.D. Chilcoat, A. Haddad, and J.W. Verbsky, Regulated Protein Secretion inTetrahymena thermophila.
T.M. Hennessey and H.G. Kuruvilla, Electrophysiology ofTetrahymena.
E.A. Wiley, C.A. Mizzen, and C.D. Allis, Isolation and Characterization ofin VivoModified Histones and an Activity Gel Assay for Identification of Histone Acetyltransferase.
C. Autexier,TetrahymenaTelomerase Activity, Purification, and Reconstruction.
E. Blackburn, D. Gilley, T. Ware, A. Bhattacharyya, K. Kirk, and H. Wang, Studying the Telomerase RNA inTetrahymena.
T.M. Gibson and D.J. Asai, Isolation and Characterization of 22S Outer Arm Dynein fromTetrahymenaCilia.
N.E. WilliamsPreparation of Cytoskeletal Fractions fromTetrahymena thermophila.
N.E. Williams, Immunoprecipitation Procedures.
O. Numato, K. Hanyu, T. Takeda, and Y. Watanabe,TetrahymenaCalcium-Binding Proteins, TCBP-25 and TCBP-23.
Manipulating Genes:
D.L. Chalker, J.G. Ward, C. Randolph, and M.-C. Yao, Microinjection ofTetrahymena thermophila.
J. Gaertig and G. Kapler, Transient and Stable DNA Transformation ofTetrahymena thermophilaby Electroporation.
P.J. Bruns and D. Cassidy-Hanley, Biolistic Transformation of Macro- and Micronuclei.
B. Hai, J. Gaertig, an M.A. Gorovsky, Knockout Heterokaryons Enable Facile Mutagenic Analysis of Essential Genes inTetrahymena.
Q. Fan, R. Sweeney, and M.-C. Yao, Creation and Use of Antisense Ribosomes inTetrahymena thermophila.
L. Yu and M.A. Gorovsky, Protein Tagging inTetrahymena.
Appendix I: Genetic Nomenclature Rules forTetrahymena thermophila.
Appendix II: Codon Usage inTetrahymena thermophila.
Chapter References.
Index.
Volumes in Series.