The Inflammasome: Methods and Protocols by Christine M. De NardoThe Inflammasome: Methods and Protocols by Christine M. De Nardo

The Inflammasome: Methods and Protocols

EditorChristine M. De Nardo, Eicke Latz

Hardcover | July 13, 2013

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This Methods in Molecular Biology book offers methods for studying inflammasome function, including generation of inflammasome stimuli, monitoring of caspase-1 activity and processing, activation of IL-1? cytokines, plus lab protocols, material lists and tips.
Title:The Inflammasome: Methods and ProtocolsFormat:HardcoverDimensions:215 pagesPublished:July 13, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627035222

ISBN - 13:9781627035224

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Table of Contents

1. Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary MicrogliaMareike Schnaars, Hannes Beckert, and Annett Halle 2. Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPPClara Westwell-Roper, Aisling Dunne, Man-Lyang Kim, C. Bruce Verchere, and Seth L. Masters 3. Assessment and Quantification of Crystal-induced Lysosomal Damage Peter Duewell and Eicke Latz 4. Assessment of Inflammasome Activation in Primary Human Immune CellsTheo S. Plantinga, Leo A.B. Joosten, and Mihai G. Netea 5. NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate AdjuvantsMarie Yang, Claire H.A. Hearnden, Ewa Oleszycka and Ed C. Lavelle 6. Measuring Inflammasome Activation in Response to Bacterial InfectionPetr Broz and Denise M. Monack 7. Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase ActivityManira Rayamajhi, Yue Zhang, and Edward A. Miao 8. ASC Speck Formation as a Read-out for Inflammasome ActivationAndrea Stutz, Gabor L. Horvath, Brian G. Monks, and Eicke Latz 9. Immunoblotting for Active Caspase-1Christopher Jakobs, Eva Bartok, Andrej Kubarenko, Franz Bauernfeind and Veit Hornung  10. Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1?, IL-1? and Caspase-1Katharina S. Schneider1, Christina J. Thomas, and Olaf Groß 11. Reconstituting the NLRP1 Inflammasome in vitroBenjamin Faustin and John C. Reed 12. Assessing ATP Binding and Hydrolysis by NLR ProteinsJinyao Mo and Joseph A. Duncan 13. Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and CompositionEric M. Kofoed and Russell E. Vance 14. Analysis of Microbiota Alterations in Inflammasome-deficient MiceEran Elinav, Christoph A Thaiss, and Richard A Flavell 15. Quantification of Adipose Tissue Leukocytosis in ObesityRyan Grant, Yun-Hee Youm, Anthony Ravussin, and Vishwa Deep Dixit 16. In vivo Evaluation of Neutrophil Recruitment in Response to Sterile ParticulatesTamiko Yanagida, Kaoru Orihashi and Hajime Kono