The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish

Kobo ebook | May 28, 2016

The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish
$4.69 online 
$5.19 list price save 9%

Prices and offers may vary in store

Available for download

Not available in stores

about

Master's Thesis from the year 2007 in the subject Biology - Zoology, grade: 1,0, University of Innsbruck (Zoologie), 90 entries in the bibliography, language: English, abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1/Ds-Red marked zebrafish. We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development.

Title:The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafishFormat:Kobo ebookPublished:May 28, 2016Publisher:GRIN PublishingLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:3638900274

ISBN - 13:9783638900270

Appropriate for ages: All ages

Look for similar items by category: