Therapeutic Proteins: Methods and Protocols by Vladimir VoynovTherapeutic Proteins: Methods and Protocols by Vladimir Voynov

Therapeutic Proteins: Methods and Protocols

EditorVladimir Voynov, Justin A. Caravella

Hardcover | June 28, 2012

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After the identification of a potential protein drug, the next critical step is the production of sufficient authentic material for testing, characterization, and clinical trials, which, when successful, leads to the need for robust methodologies for large-scale production, purification, characterization, viral inactivation, and continued testing of the final protein product.  Building on the valuable first edition, Therapeutic Proteins: Methods and Protocols, Second Edition aims to cover each of these key aspects of protein drug production through the contributions of authors from highly esteemed industrial and academic institutions around the world.  Emphasizing the newest developments in the field, this second edition also includes additional emphasis on discovery, including new display and screening methods as well as the design and engineering of new types of therapeutic proteins.  There is also discussion of computational and bioinformatics methods, and chapters on safety aspects of therapeutic protein development.  Written in the highly successful Methods in Molecular BiologyT format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and practical, Therapeutic Proteins: Methods and Protocols, Second Edition provides an essential resource to all scientists working in the field of therapeutic proteins.
Title:Therapeutic Proteins: Methods and ProtocolsFormat:HardcoverDimensions:513 pagesPublished:June 28, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617799203

ISBN - 13:9781617799204

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Table of Contents

1. Therapeutic Proteins            Dimiter S. Dimitrov2. Synthetic Antibody Libraries             Bryce Nelson and Sachdev S. Sidhu3. The Construction of 'Phylomer' Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions             Nadia Milech and Paul Watt4. Ribosome Display and Screening for Protein Therapeutics             Damjana Kastelic and Mingyue He5. Yeast Display of Engineered Antibody Domains             Qi Zhao, Zhongyu Zhu, and Dimiter S. Dimitrov 6. Design, Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains             Rui Gong, Weizao Chen, and Dimiter S. Dimitrov7. Engineering of Affibody Molecules for Therapy and Diagnostics             Joachim Feldwisch and Vladimir Tolmachev8. Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination             Karl J.M. Hanf9. Design and Generation of DVD-IgTM Molecules for Dual-Specific Targeting             Enrico DiGiammarino, Tariq Ghayur, and Junjian Liu10. Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris             Steve Schoonooghe 11. Use of E. coli for the Production of a Single Protein             Lili Mao and Masayori Inouye 12. Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli             Brent L. Nannenga and François Baneyx 13. Transient Expression Technologies: Past, Present, and Future             Sabine Geisse and Bernd Voedisch14. Stable Transfection Pools for Large Quantity of Protein Production             Jianxin Ye 15. Mammalian Stable Expression of Biotherapeutics             Thomas Jostock and Hans-Peter Knopf16. Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed             Cory L. Nykiforuk and Joseph G. Boothe17. Methods for Chromatographic Removal of Endotoxin             Adam J. Lowe, Cameron L. Bardliving, and Carl A. Batt18. Effectiveness of Various Processing Steps for Viral Clearance of Therapeutic Proteins: Database Analyses of Commonly-Used Steps             Dana Cipriano, Michael Burnham, and Joseph V. Hughes 19. High-Throughput Quantitative N-Glycan Analysis of Glycoproteins             Margaret Doherty, Ciara A. McManus, Rebecca Duke, and Pauline M. Rudd20. High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris             Sujatha Gomathinayagam, Erik Hoyt, Alissa M. Thompson, Eric Brown, Khanita Karaveg, Stephen Hamilton, and Huijuan Li21. Databases and Tools in Glycobiology             Natalia V. Artemenko, Andrew G. McDonald, Gavin P. Davey, and Pauline M. Rudd22. Characterization of PEGylated Biopharmaceutical Products by LC/MS and LC/MS/MS             Lihua Huang and P. Clayton Gough23. Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry             Jennifer Zhang and Viswanatham Katta24. Monitoring of Subvisible Particles in Therapeutic Proteins             Satish K. Singh and Maria R. Toler25. Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) for Elucidating Protein Aggregation Mechanisms             Erinc Sahin and Christopher J. Roberts26. Computational Methods to Predict Therapeutic Protein Aggregation             Patrick M. Buck, Sandeep Kumar, Xiaoling Wang, Neeraj J. Agrawal, Bernhardt L. Trout, and Satish K. Singh27. Coarse-Grained Simulations of Protein Aggregation             Troy Cellmer and Nicolas L. Fawzi28. Chitosan-Based Nanoparticles as Delivery Systems of Therapeutic Proteins             Pedro Fonte, José Carlos Andrade, Vítor Seabra, and Bruno Sarmento29. Challenges in the Development and Manufacturing of Antibody-Drug Conjugates             Laurent Ducry            Lihua Huang and P. Clayton Gough