Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State by Joachim Frank

Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological…

byJoachim Frank

Paperback | February 15, 2006

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Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approachescurrently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion ofbiomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.

About The Author

Joachim Frank is an Investigator at the Howard Hughes Medical Institute. He is also Professor of Biomedical Sciences and Adjunct Professor of Biology at the State University of New York at Albany, Adjunct Professor of Biochemistry and Molecular Biophysics at Columbia University, Research Professor of Cell Biology at New York State Uni...
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Title:Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological…Format:PaperbackDimensions:432 pages, 5.98 × 9.09 × 0.91 inPublished:February 15, 2006Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0195182189

ISBN - 13:9780195182187

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Table of Contents

1. Introduction2. Electron Microscopy of Macromolecular Assemblies3. Two-Dimensional Averaging Techniques4. Multivariate Data Analysis and Classification of Images5. Three-Dimensional Reconstruction6. Interpretation of Three-Dimensional Images of MacromoleculesAppendix 1: Some Important Definitions and TheoremsAppendix 2: Profiles, Point-Spread Functions, and Effects of Commonly Used Low-Pass FiltersAppendix 2: Bibliography of MethodsAppendix 2: Bibliography of StructuresAppendix 2: Special Journal Issues on Image Processing Techniques