Transcription Factors: A Practical Approach

Paperback | April 8, 1999

EditorDavid Latchman

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Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to themethods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomicbinding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. Thenext two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library. Chapter 6 deals with identification of transcription factors based on sequence homology analysis by bothexperimental screening and database searches. Chapter 7 is a new chapter that describes methods of identifying the target genes of a previously uncharacterized factor. The next chapters deal with analysis of transcription factor function. Chapter 8 deals with general techniques, and then thefollowing chapters cover the specialized techniques of in vitro transcription assays using transcriptionally active nuclear extracts derived from rat brain, and analysis of the effect of transcription factors on chromatin structure. The final chapter describes methods for detecting thephosphorylation and glycosylation state of transcription factors.

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From the Publisher

Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to themethods currently available to characterize...

David Latchman is at University College London.

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Format:PaperbackPublished:April 8, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636966

ISBN - 13:9780199636969

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Table of Contents

1. The DNA mobility shift assay2. Footprint analysis of DNA-protein complexes in vitro and in vivo3. In vitro transcription and characterization of transcription factors4. Purification and cloning of DNA binding transcription factors5. Cloning transcription factors from a cDNA expression library6. Cloning transcription factors by sequence similarity7. Identification of target genes for a transcription factor by genomic binding site cloning8. Analysis of cloned factors9. Neuronal promoter analysis by in vitro transcription using nuclear extracts from rat brain10. Preparation of chromatin templates for transcription studies11. Analysis of transcription factor modificationsAppendix: Suppliers of specialist items

Editorial Reviews

'..this book is excellent value for money and is of the high quality expected of books from the series...' Cell Biology International..'