Transcription Regulation in Prokaryotes by Rolf WagnerTranscription Regulation in Prokaryotes by Rolf Wagner

Transcription Regulation in Prokaryotes

byRolf Wagner

Paperback | May 15, 2000

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Transcription is part of the process of gene expression and is very different in prokaryotes and eukaryotes. In prokaryotic cells the synthesis reactions of DNA, RNA and proteins are intricately coupled as there are no subcellular compartments. Thus replication, transcription and translationoccur simultaneously without local separation and the regulations of each is inter-related. In bacteria, regulation is most important at the transcription stage of the biosynthetic process, and changes in the transcriptional efficiency account for major differences in the frequency of RNA formationand gene expression. This textbook has been written by an authority in the field for advanced students and postgraduates. Both clear and concise, there is an emphasis on transcription in bacterial and phage expression systems, a key area of study and research in molecular biology. Many generalmechanisms and principles emerge from studies of prokaryotic transcription complexes that help build an understanding of regulation in higher organisms. Taking a biophysical approach, the text draws together elements of molecular biology, genetics, structural biology and biochemistry to provide an invaluable summary of the field of prokaryotic transcription regulation for students in molecular biology and related areas of study such as microbiologyand biotechnology. Additional content information: this book includes -new data on the composition and architecture of RNA polmerase - new findings on sigma- and anti-sigma- factors -a description of promoter elements underlining the importance of upstream and downstream flanking sequences -detailed kinetic description of the initiation process -new data on the function of DNA curvature and topology on transcription -general mechanisms of repression -positive regulation -termination, anti-termination and attenuation mechanisms -implications of the nascent RNA structure in regulation -stringent control mechanisms and global networks -comparative chapter for parallel and divergent mechanisms in prokaryotic and eukaryotic transcription regulation -modern methods of analysis and new technical developments with selected examples
Prof Rolf Wagner runs the Institute of Physical Biology and teaches part of the molecular biology undergraduate course at the University of Dusseldorf. He has been involved in active research and teaching of prokaryotic transcription control for over ten years.
Title:Transcription Regulation in ProkaryotesFormat:PaperbackPublished:May 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0198503547

ISBN - 13:9780198503545

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Table of Contents

1 General introductionFlow of genetic informationDistinctive features of prokaryotic gene regulationBiochemical nature of the transcription reactionWhat makes RNA transcription different from DNA replication?The products of transcription2 The "players" or cellular components necessary for transcription2.1 Transcription Start sites: the promoter2.2 RNA polymerase2.3 Alternative ( factors2.4 The 3-dimensional structure of RNA polymerase2.5 Is RNA polymerase modified during different stages of transcription?Initiation of transcription3.1 Promotor location3.2 The closed RNA polymerase-promotor complex3.3 Formation of open complexes3.4 Binding of substrate NTPs the ternary complexesPromotor clearance and the formation of elongating complexesThe thermodynamics and kinetics of transcription initiationTranscription elongation4.1 The architecture of elongating complexes what is known about the different functional site?4.2 The rate of elongation4.3 RNA polymerases during transcription4.4 Transcript cleavage factors'Traffic problems' at the bacterial genome inevitable collisions between transcription and replication machineriesTermination of transcription5.1 Factor-independent termination5.2 Factor dependent termination5.3 Attenuation of transcription5.4 AntiterminationThe role of DNA structure in transcription regulation6.1 Is DNA methylation involved in regulation?6.2 Effects of DNA curvature on transcription inhibition6.3 DNA supercoiling and transcriptionRegulation by transcription factors7.1 Repressors and operators the classical model7.2 The modular structure of transcription factors7.3 LysR a representative of a large transcription factor family7.4 DNA loops regulation through dynamic structuresregulation by DNA structuring proteinsTwo component regulatory systemsRegulatory networks8.1 The SOS response8.2 The heat-shock response8.3 Stationary phase control8.4 The Lrp regulonThe stringent controlGrowth rate regulationLessons from a many-fold regulated system the synthesis of ribosomal RNA

Editorial Reviews

'a superb, compact yet comprehensive, treatise on the regulation of gene expression, principally but not exclusively, in E.Coli and its phage ... A must for all students at undergraduate or postgraduate level and also for reseachers of eukaryotic transcription who need reminding of a fewparadigms' Aslib