Transgenic Maize: Methods and Protocols by M. Paul ScottTransgenic Maize: Methods and Protocols by M. Paul Scott

Transgenic Maize: Methods and Protocols

byM. Paul Scott

Hardcover | March 5, 2009

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As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular BiologyT series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.

Title:Transgenic Maize: Methods and ProtocolsFormat:HardcoverDimensions:188 pagesPublished:March 5, 2009Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1934115495

ISBN - 13:9781934115497

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Table of Contents

Part I: Introduction1. Design of Gene Constructs for Transgenic MaizeDong LiuPart II: Transformation Methods2. Transient Expression of GFP in Immature Seed TissuesColin T. Shepherd3. Biolistic Gun Mediated Maize Genetic TransformationKan Wang and Bronwyn Frame4. Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus CallusVladimir Sidorov and David Duncan5. Whiskers-Mediated Maize TransformationJoseph F. Petolino and Nicole L. ArnoldPart III: Transgenic Maize in Research6. Methods for Generation and Analysis of Fluorescent Protein Tagged Maize LinesAmitabh Mohanty, Yan Yang, Anding Luo, Anne Sylvester, and David Jackson7. Use of Transgene-Induced RNAi to Regulate Endogenous Gene ExpressionKaren M. McGinnis8. Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion SitesGuo-Ling Nan and Virginia WalbotPart IV: Analysis of Transgenic Plants9. Non-Radioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic MaizeGuo-Ling Nan and Virginia Walbot10. Tissue-Print Immuno-Detection of Transgene Products in Endosperm for High-Throughput Screening of SeedsM. Paul Scott11. Determination of Transgene Copy Number by Real-Time Quantitative-PCRColin T. Shepherd, Adrienne N. Moran Lauter, and M. Paul ScottPart V: Breeding with Transgenes12. Herbicide Resistance Screening AssayJoan M. Peterson13. Characterizing Transgene InheritanceEarl H. Bicar14. Backcross BreedingKarla E. Vogel Appendix A: Sample APHIS Notification of Release of Regulated MaterialAppendix B: Sample SOP for Field Release

Editorial Reviews

From the reviews:"This book is number 526 in the popular 'Methods in Molecular Biology' series, which is renowned for its targeted protocol chapters, each with photographs, recipes and a set of notes for additional practical guidance and to avoid common pitfalls. . There is a lot to like about this collection of practical chapters, which represent an excellent investment for the applied maize researcher, who could be a student doing an undergraduate project through to a professional scientist in a dedicated transformation laboratory." (Huw D. Jones, Annals of Botany, Vol. 109 (7), June, 2012)