Transplantation Immunology: Methods and Protocols by Andrea A. ZacharyTransplantation Immunology: Methods and Protocols by Andrea A. Zachary

Transplantation Immunology: Methods and Protocols

EditorAndrea A. Zachary, Mary S. Leffell

Hardcover | June 18, 2013

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After decades of research in clinical transplantation,  new techniques have been developed that permit a further understanding of the  immune mechanisms underlying immune recognition of allografts and a more accurate and  thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology.  The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research.  Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms,  as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients.  Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research.
Title:Transplantation Immunology: Methods and ProtocolsFormat:HardcoverDimensions:355 pages, 10 × 7.01 × 0.01 inPublished:June 18, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627034927

ISBN - 13:9781627034920

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Table of Contents

PART I: Overview Chapters1. AlloreactivitySidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey 2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody Binding and Mechanisms of InjuryNicole M. Valenzuela and Elaine F. Reed 3. Cell Mediated RejectionKathryn J Wood, Masaaki Zaitsu and Ryoichi Goto 4. Transplantation ToleranceColin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg 5. Composite Tissue TransplantationGerald Brandacher 6. Transplantation of the Sensitized Patient: Histocompatibility TestingRobert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary 7. Thoracic Organ Transplantation: Laboratory MethodsJignesh K. Patel and Jon A. Kobashigawa  PART II: Methods Chapters  8. HLA Typing by Sequence Specific PrimersMike Bunce, Biofortuna and Ben Passey, Biofortuna  9. Human Leukocyte Antigen (HLA) Typing By DNA SequencingAna Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley 10. Next-Generation HLA Sequencing using the 454 GS FLX System Elizabeth A. Trachtenberg and Cherie L. Holcomb 11. Chimerism Testing by Quantitative PCR  Using Indel MarkersKetevan Gendzekhadze, Laima Gaidulis, and David Senitzer 12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR)Raja Rajalingam and Elham Ashouri 13. Complement-dependent Cytotoxicity CrossmatchJeremy Ryan Peña, Donna Fitzpatrick, and Susan L. Saidman 14. Lymphocyte Crossmatching by Flow CytometryRobert A. Bray 15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific AntibodiesJohn D Smith and  Marlene L Rose 16. C1q Assay for the Detection of Complement Fixing Antibody to HLA AntigensGe Chen and Dolly B. Tyan 17. Tetramer Staining for the Detection of HLA-specific B cellsDonna P. Lucas, Mary S. Leffell, and Andrea A. Zachary 18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral BloodAnnette M. Jackson, Donna P. Lucas, Jessica L. Badders 19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in TransplantationDuska Dragun 20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA)Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar 21. Cylex  ImmuKnow Cell Function AssayAdriana Zeevi and John Lunz 22. Detection of Intracellular CytokinesNancy L. Reinsmoen and Chih-Hung Lai 23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant RecipientsGeorge Vlad and Nicole Suciu-Foca 24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISATara K. Sigdel, and Minnie. M. Sarwal 25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian