Tumor Marker Protocols by Margaret HanausekTumor Marker Protocols by Margaret Hanausek

Tumor Marker Protocols

EditorMargaret Hanausek, Zbigniew Walaszek

Paperback | November 9, 2010

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A much-needed collection of cutting-edge methods for the detection and discovery of new cancer markers, particularly molecular tumor markers. Each method is fully described by a hands-on expert who regularly uses it in the laboratory and has perfected it to a high degree of ready reproducibility. Special emphasis is given to statistical considerations in the analysis of tumor markers, recent discoveries in the field of serum and tissue marker proteins, cytogenetic markers, molecular markers, and markers for the early detection of cancer. The well-honed procedures detailed here will enable cancer researchers and clinical oncologists alike to realize the promise of these powerful new diagnostic strategies for the detection, therapy, and successful prevention of cancer.
Title:Tumor Marker ProtocolsFormat:PaperbackDimensions:516 pages, 9.02 × 5.98 × 0 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617370193

ISBN - 13:9781617370199

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Table of Contents

Part I. Multiple Markers: Principles and GuidelinesIntegrating Multiple Clinical Tests to Increase Predictive PowerHarry B. BurkeStatistical Considerations in the Analysis of Tumor MarkersDennis A. JohnstonSelection and Development of Biomarkers for Bladder CancerGeorge P. Hemstreet III, Robert E. Hurst, and Rebecca B. BonnerClinical Application of Tissue and Serum Markers in Breast CancerGordon F. Schwartz and Roland SchwartingPart II. Immunodiagnosis of Cancer: Serum and Tissue MarkersSerum and Tissue Biomarkers in the Prognosis and Treatment of Breast CancerTina J. Heiken and Rajeshwari R. MehtaThe Oncofetal Protein p65 in Breast Cancer DetectionMargaret Hanausek, Zbigniew Walaszek, Ute Sherman, and Jerzy T. KlijanienkoDetermination of Tumor Ferritin Concentration in Breast CancerJonathan F. Head and Robert L. ElliottImmunodiagnosis of Childhood MalignanciesDavid M. Parham and Hallie HoltImmunohistochemical Evaluation of Biomarkers in Prostatic and Colorectal Neoplasia: Principles and GuidelinesWilliam E. Grizzle, Russell B. Myers, Upender Manne, and Sudhir SrivastavaFactors Affecting Immunohistochemical Evaluation of Biomarker Expression in NeoplasiaWilliam E. Grizzle, Russell B. Myers, Upender Manne, Cecil Rob Stockard, Lualhati E. Harkins, and Sidhir SrivastavaInstrumentation, Accuracy, and Quality Control Issues in Development of Quantitative Fluorescence-Image Analysis (QFIA)Rebecca B. Bonner, Rubert E. Hurst, Jian Yu Rao, and George P. HemstreetPart III. Cytogenetic MarkersCytogenetics as a Diagnostic Aid for Childhood Hematologic Disorders: Conventional Cytogenetic Techniques, Fluorescence In Situ Hybridization, and Comparative Genomic HybridizationSusanna C. Raimondi, Susan Mathew, and Ching-Hon PuiPreparation of Metaphase Chromosomes for Cytogenetic AnalysisKevin A. Hahn and Penny K. RiggsChromosome Staining and Banding TechniquesKevin A. Hahn and Penny K. RiggsFluorescence In Situ Hybridization to ChromosomesPenny K. Riggs and Kevin A. HahnDetection of Rearrangements in the bcl-2 Gene Using the Polymerase Chain ReactionRuth W. Craig, Dorothy R. Belloni, Ernest S. Kawasaki, and Norman B. LevyPart IV. Molecular MarkersApplications of Tissue Microdissection in Molecular Pathology: Principles and GuidelinesMichael R. Emmert-Buck, Irina A. Lubensky, Rodrigo F. Chuaqui, Larisa V. Debelenko, Cathy D. Vocke, Maria J. Merino, Paul H. Duray, W. Marston Linehan, Lance A. Liotta, and Zhengping ZhuangEWS Gene Fusions as Diagnostic Markers in Sarcomas: Principles and GuidelinesMarc Ladanyip53 Detection in Breast CancerPenelope L. Davis and J. Dirk IglehartUse of the Polymerase Chain Reaction Technique to Detect the t(14;18) Translocation in Lymphoid TissueMaryalice Stetler-Stevenson and Megan S. LimDetection of ras Gene Mutations Using Oligonucleotide Ligation TechnologyFaye A. EggerdingDetection of Prostate-Cancer Cells in Blood and Bone Marrow by RT-PCRRobert L. Vessella and Eva CoreyQuantitative, Competitive RT-PCR Analysis of Biomarkers in the Study of NeoplasiaRichard D. Hockett Jr., Miriam D. Rogers, and Sudhir SrivastavaDifferential Display to Define Molecular Markers and Genes That Mediate MalignancyEdward J. Pavlik, Katherine Nelson, Suseela Srinivasan, Thomas L. Johnson, and Paul D. DePriestPart V. Miscellaneous Markers and Early Detection of CancerTransforming Growth Factor Beta: A Plasma Tumor MarkerFeng-Ming Kong, Mitchell S. Anscher, and Randy L. JirtleAnti-HMdU Autoantibodies in Human Sera as a Biomarker of Cancer RiskKrystyna Frenkel and Jerzy KarkoszkaA Scientific Basis for Cancer Prevention: Defining the Role of Individual Cytosolic GST IsozymeSanjay K. Srivastava and Sanjay AwasthiAberrant Crypt Foci System to Study Cancer Preventative Agents in the Colon: Principles and GuidelinesRanjana P. BirdStrain-Dependent Differences in the Expression of the Oncofetal Protein p65 in Mice Susceptible and Resistant to Chemical CarcinogenesisJanusz Szemraj, Margaret Hanausek, Zbigniew Walaszek, and Alan K. AdamsIndex