Type 2 Diabetes: Methods and Protocols by Claire StockerType 2 Diabetes: Methods and Protocols by Claire Stocker

Type 2 Diabetes: Methods and Protocols

EditorClaire Stocker

Paperback | November 30, 2011

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Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular BiologyT series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.
Title:Type 2 Diabetes: Methods and ProtocolsFormat:PaperbackDimensions:288 pagesPublished:November 30, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617794929

ISBN - 13:9781617794926

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Table of Contents

1) Selecting the "Right" Mouse Model for Metabolic Syndrome and Type 2 Diabetes ResearchEdward H. Leiter2) Nutritional Models of Type 2 Diabetes MellitusBeverly Sara Muhlhausler3) The Isolation and Purification of Rodent Pancreatic Islets of LangerhansJacqueline F. O'Dowd4) The Measurement of Insulin Secretion from Isolated Rodent Islets of LangerhansAnna L. Nolan and Jacqueline F. O'Dowd5) The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ß-Cell LinesNoel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell6) In Vitro Culture of Isolated Islets of Langerhans: Analysis of FunctionAnna L. Nolan7) Single Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine CellsDany Muller, Peter M. Jones, and Shanta J. Persaud8) Laser Capture Microdissection of Human Pancreatic b -Cells and RNA Preparation for Gene Expression Profiling Lorella Marselli, Dennis C. Sgroi, Susan Bonner-Weir, and Gordon C. Weir9) In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like CellsWan-chun Li10) The Measurement of GLUT4 Translocation in 3T3-L1 AdipocytesNicky Konstantopoulos and Juan Carlos Molero-Navajas11) Pancreatic Remodeling: Beta Cell Apoptosis, Proliferation and Neogenesis and the Measurement of Beta Cell Mass and of Individual Beta Cell SizeEduard Montanya and Noèlia Téllez12) Morphology of Pancreatic Islets: A Time Course of Pre-Diabetes in Zucker Fatty RatsPetra Augstein and Eckhard Salzsieder13) Fluorescent Immunohistochemistry and in situ Hybridization Analysis of PancreasXiuli Wang, Shundi Ge, and Gay M. Crooks14) The Measurement of Insulin Secretion Using Pancreas Perfusion in the RodentEdward T. Wargent15) Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In VivoJason K. Kim16) Gene Expression Analysis in Diabetes ResearchPeter White and Klaus H. Kaestner17) Gene Expression Mining in Type 2 Diabetes ResearchDonald R. Dunbar

Editorial Reviews

From the reviews: "This book provides in 17 chapters laboratory protocols used in diabetes research. It describes methods using new technologies in studying pancreatic beta cells. For research diabetologists." (Pediatric Endocrinology Reviews, Vol. 7 (4), June, 2010)"Written for those engaged in basic science research who have an interest in expanding their repertoire of techniques for the study of diabetes. . a credible source of information for those seeking experimental advice. . an excellent compilation of techniques for the laboratory study of diabetes, particularly those who are interested in studying pancreatic beta cells. Many techniques are covered, with up-to-date and relevant references as well as step-by-step instructions for performing them. The book is a useful addition to any diabetes researcher's bookshelf." (Robert Michael Sargis, Doody's Review Service, July, 2010)