Understanding Pcr: A Practical Bench-top Guide

Paperback | October 28, 2016

bySarah Maddocks, Rowena Jenkins

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and ...

Dr Sarah E. Maddocks is a Lecturer of Microbiology at Cardiff Metropolitan University, UK. She received a PhD from the University of Reading in 2005 and worked on bacterial virulence before embarking upon investigations into the host-pathogen relationship during infection. Her main research interests involve the study of wound colonisa...
Format:PaperbackDimensions:98 pages, 8.75 × 6.35 × 0.68 inPublished:October 28, 2016Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128026839

ISBN - 13:9780128026830

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Table of Contents

Chapter 1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction

Chapter 2. Designing and Ordering Your Polymerase Chain Reaction Primers

Chapter 3. Analyzing Your Template DNA and/or PCR Product

Chapter 4. Quantitative PCR: Things to Consider

Chapter 5. Carrying Out Q-PCR

Chapter 6. Using PCR for Cloning and Protein Expression

Chapter 7. Polymerase Chain Reaction for Knocking Out Genes