Understanding Pcr: A Practical Bench-top Guide

Paperback | November 3, 2016

bySarah Maddocks, Rowena Jenkins

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and ...

Dr Sarah E. Maddocks is a Lecturer of Microbiology at Cardiff Metropolitan University, UK. She received a PhD from the University of Reading in 2005 and worked on bacterial virulence before embarking upon investigations into the host-pathogen relationship during infection. Her main research interests involve the study of wound colonisa...
Format:PaperbackDimensions:98 pages, 8.75 × 6.35 × 0.68 inPublished:November 3, 2016Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128026839

ISBN - 13:9780128026830

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Table of Contents

1. Things to consider before planning your first PCR
2. Designing your primers
3. Extracting DNA/RNA
4. Choosing the basic parameters
5. Analysing your first PCR
6. Troubleshooting your first PCR
7. RT-PCR - things to consider
8. Carrying out your first RT-PCR
9. Analysing your first RT-PCR
10. Troubleshooting your RT-PCR