Vaccine Protocols by Andrew P. RobinsonVaccine Protocols by Andrew P. Robinson

Vaccine Protocols

EditorAndrew P. Robinson, Martin P. Cranage, Michael J. Hudson

Paperback | November 10, 2010

Pricing and Purchase Info

$260.34 online 
$297.95 list price save 12%
Earn 1,302 plum® points

Prices and offers may vary in store


In stock online

Ships free on orders over $25

Not available in stores


This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines, and vaccines based on inactivated toxins, peptides, and polysaccharide conjugates. New to this edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. There are also chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials.
Title:Vaccine ProtocolsFormat:PaperbackDimensions:426 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373826

ISBN - 13:9781617373824

Look for similar items by category:


Table of Contents

Overview of VaccinesGordon AdaTemperature-Sensitive Mutant VaccinesCraig R. PringleLive Viral Vectors: Construction of a Replication-Deficient Recombinant AdenovirusAnthony R. FooksLive Viral Vectors: Vaccinia VirusCaroline Staib and Gerd SutterLive Viral Vectors: Semliki Forest VirusGunilla B. Karlsson and Peter J. LiljeströmDevelopment of Attenuated Salmonella Strains That Express Heterologous AntigensFrances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londoño-Arcila, and Gordon DouganExpression and Delivery of Heterologous Antigens Using Lactic Acid BacteriaMark A. Reuter, Sean Hanniffy, and Jerry M. WellsSynthetic PeptidesMichael J. FrancisGenetic Detoxification of Bacterial ToxinsMariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, and Rino RappuoliPreparation of Polysaccharide-Conjugate VaccinesCarla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret, Karen M. Reddin, and Jan T. PoolmanAdjuvant Formulations for Experimental VaccinesDuncan E. S. Stewart-TullIncorporation of Immunomodulators into Plasmid DNA VaccinesLinda S. Klavinskis, Philip Hobson, and Andrew WoodsMicroencapsulation of Vaccine AntigensDavid H. JonesLyophilization of Vaccines: Current TrendsGerald D. J. AdamsStimulation of Mucosal ImmunityDavid J. M. Lewis and Christopher M. M. HaywardInduction and Detection of T-Cell ResponsesKingston H. G. MillsConstruction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune ResponsesRachel V. Samuel and Tomás HankeAssessment of Functional Antibody ResponsesRay Borrow and Paul BalmerThe Use of Complete Genome Sequences in Vaccine DesignNigel J. Saunders and Sarah ButcherSevere Combined Immunodeficient (SCID) Mice in Vaccine AssessmentMichael J. DennisClinical TrialsPaddy Farrington and Elizabeth MillerAssuring the Quality and Safety of Vaccines: Regulatory Expectations for Licensing and Batch ReleaseElwyn Griffiths and Ivana KnezevicDNA Vaccination: An UpdateDouglas B. LowrieFrom Vaccine Research to Manufacture: A Guide for the ResearcherNigel Allison and Howard S. TranterIndex

Editorial Reviews

"This manual serves as a valuable guide to the process of designing, producing and assessing vaccines used against infectious agents including viruses, bacteria and parasites. This book would most benefit scientists involved in the research and development associated with vaccine technology. It also would be most useful to those new to the field of vaccine formulation and production. Health and science libraries supporting research institutions, government agencies, academic and corporate entities involved in developing or producing vaccines will find this a valuable addition to the collection." -E-STREAMSFrom reviews of the first edition..."...cover[s] concepts and protocols applicable to a wide range of vaccines and infectious diseases and cancer...brings together many protocols of value to research and development scientists involved in the vaccine technology area." -Immunology and Cell Biology"...a valuable basic reference manual of vaccine related laboratory procedures...strongest in the area of vaccine development and delivery technology...especially valuable to those new to the field of vaccine development and formulation." -Doody's Health Sciences Book Review Journal