Vascular Disease: Molecular Biology and Gene Transfer Protocols by Andrew H. BakerVascular Disease: Molecular Biology and Gene Transfer Protocols by Andrew H. Baker

Vascular Disease: Molecular Biology and Gene Transfer Protocols

EditorAndrew H. Baker

Paperback | November 9, 2010

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Andrew Baker and a noted panel of expert investigators describe today's most powerful molecular methods for investigating the pathogenesis of vascular disease. These detailed, easy-to-follow techniques range from methods that have been used successfully to identify specific mutations involved in cardiovascular disorders, to those for transferring genes associated with cardiovascular disease into various vascular cell types by in vitro and in vivo routes. There are methods to identify novel genes and generate full-length cDNAs, to study gene transcription and promoter activity easily and effectively, and to ascertain precisely gene expression levels within the individual cell types in different pathophysiological conditions. Vascular Disease: Molecular Biology and Gene Therapy Protocols offers today's vascular biologist and gene therapist an unprecedented ability to study the pathogenesis of vascular disease and readily to probe the potential for gene-based therapies.
Title:Vascular Disease: Molecular Biology and Gene Transfer ProtocolsFormat:PaperbackDimensions:456 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371556

ISBN - 13:9781617371554

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Table of Contents

Part I. Genetics in Vascular DiseaseDetection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism AnalysisShu Ye and Adriano M. HenneyAnalysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex AnalysisAna Cenarro, Fernando Civeira, and Miguel PocoviRadiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted GenesJoseph M. Miano, Emilio Garcia, and Ralf KrahePart II. Isolation of Genes Expressed in Vascular TissueEfficient Extraction of RNA from Vascular TissueCatherine F. Townsend, Christopher M. H. Newman, and Sheila E. FrancisPreparation of cDNA Libraries from Vascular CellsMark E. Lieb and Mark B. TaubmanScreening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular CellsCsilla Csortos, Virginie Lazar, and Joe G. N. GarciaCloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCRRong-Fong ShenUse of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic PlaquesKerry Tyson and Catherine ShanahanThe Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular CellsPaul J. AdamIdentification of Novel Protein Kinases in Vascular CellsIan Zachary, Spiros Servos, and Barbara HerrenPart III. Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular CellsPrimer Extension Analysis to Map Transcription Start Sites of Vascular GenesYutaka Kitami and Kunio HiwadaUse of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle CellsRosalind P. FabunmiNuclear Run-On Assay to Study Gene Transcription in Vascular CellsUlrich Laufs and James K. LiaoElectromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle CellsTodd BourcierPart IV. Molecular Analysis of mRNA Expression in Vascular CellsMeasurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) AnalysisJoachim FruebisNorthern Blot Analysis to Quantify Gene Expression in Vascular DiseasesMingyi Chen and Tatsuya SawamuraLocalization of Gene Expression in Human Atherosclerotic Lesions by In Situ HybridizationLee D. K. Buttery and Julia M. PolakRNase Protection Assays for Quantification of Gene Expression in Vascular TissueNengyu Yang, Shuli Wang, and James E. FaberPart V. Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and TissueDetection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick End Labeling (TUNEL)Mark Kockx, Johannes Muhring, and Michiel KnaapenIn Vitro Detection of Apoptosis in Isolated Vascular CellsShiu-Wan Chan and Martin R. BennettMolecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence AnalysisRachel Johnatty, Giulio Gabbiani, and Pascal NeuvillePart VI. In Vitro Methods to Express Foreign Genes in Vascular CellsSimple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular CellsStuart A. Nicklin and Andrew H. BakerGeneration of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular CellsCarmel M. LynchHemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular CellsYoshikazu Yonemitsu and Yasufumi KanedaHigh-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial TransfectionTakamune Takahashi, Keiko Takahashi, and Thomas O. DanielGene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable MarkerJacques Pouysségur and Danièle RouxUse of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle CellsTrevor D. Littlewood and Stella A. PelengarisEmbryonal Stem (ES) Cell-Derived Macrophages: A Cellular System that Facilitates the Genetic Dissection of Macrophage FunctionKathryn J. Moore and Mason W. FreemanDelivery of Recombinant Adenoviruses to Human Saphenous VeinSarah J. George and Andrew H. BakerPart VII. In Vivo Vascular Gene Transfer ProtocolsAn In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of NeovascularizationMaurizio C. Capogrossi and Antonino PassanitiEfficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries In VivoMichael-Christopher Keogh and Daxin ChenGene Delivery to Rabbit Arteries Using the Collar ModelMikko O. Hiltunen, Mikko P. Turunen, and Seppo Ylä-HerttualaDelivery of Antisense Oligonucleotides to the Vascular WallCathy M. Holt, Julian Gunn, Darren Lambert, David C. Cumberland, and David CrossmanLocal Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In VivoClare M. Dollery and Jean R. McEwanIndex.

Editorial Reviews

"...The chapters are clearly written so that complex techniques are reduced to their individual components...this book will be a major asset to a molecular biology laboratory involved in vascular research."-Atherosclerosis"This collection of very detailed molecular biology protocols was compiled...The first five parts present protocols that are designed to identity and characterize genes in cardiovascular disease, including methods for detecting specific gene mutation, identifying new genes, characterizing the levels and regulation of expression of the new genes, and apoptotic methods. The final two parts contain in vivo and in culture gene transfer protocols used in cardiovascular disease and protocols for clinical gene therapy....an excellent reference for molecular biologists in the field of vascular disease." - Quarterly Review of Biology"...the authors of the individual chapters have made every effort to relate their particular techniques to the study of vascular tissues....the editor has assembled a broad base of well established authors to describe their protocols....this is a good book....this book may prove worthwhile in helping to ease the load on the typical physician-scientist trying to care for patients and at the same time direct his or her laboratory research program."- Texas Heart Institute Journal"The book is the result of the contribution of distinguished experts in the field. Each chapter of the book is introduced by a theoretical summary of the goal of the technique with excellent diagrams followed by the material used, the basic procedures and the methods. Critical notes and references end each chapter. This important publication directed to molecular biologists covers the latest aspects of molecular procedures for the study of cardiovascular diseases and confirms, if necessary, the irreversible revolution in cardiovascular research."- Acta Cardiologica