Viral Applications of Green Fluorescent Protein: Methods and Protocols by Barry W. HicksViral Applications of Green Fluorescent Protein: Methods and Protocols by Barry W. Hicks

Viral Applications of Green Fluorescent Protein: Methods and Protocols

byBarry W. Hicks

Hardcover | April 1, 2009

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Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyT series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

Title:Viral Applications of Green Fluorescent Protein: Methods and ProtocolsFormat:HardcoverDimensions:357 pagesPublished:April 1, 2009Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1934115878

ISBN - 13:9781934115879

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Table of Contents

1 Using Fluorescent Proteins to Study Poxvirus MorphogenesisBrian M. Ward2 Application of Reporter Gene Imaging for Studying Human Embryonic Stem Cells in Living SubjectsKazim H. Narsinh, Feng Cao, and Joseph C. Wu3 Use of a GFP-PML-expressing Cell Line as a Biosensor for Human Cytomegalovirus InfectionTomonori Ueno and Kiyoko Ogawa-Goto4 Dual-Color Imaging of Tumor AngiogenesisRobert M. Hoffman5 Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous SystemAlan R. Harvey, Erich Ehlert, Joris de Wit, Eleanor S. Drummond, Margaret A. Pollett, Marc Ruitenberg, Giles W. Plant, Joost Verhaagen, and Christiaan Levelt6 Expression of EGFP by Adenovirus-mediated Gene Transfer in the Central Nervous SystemRyohei Tomioka7 Adenovirus-mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac MyocytesAndreas Rinne, Christoph Littwitz, Kirsten Bender, Marie-Cecile Kienitz, and Lutz Pott8 Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry Michael Grusch, Annemarie Losert, Andreas Lackner, Alev Deli, Irene Herbacek, and Klaus Holzmann9 Production of Multicistronic HIV-1 Based Lentiviral VectorsNachimuthu Chinnasamy, James Shaffer, and Dhanalakshmi Chinnasamy10 Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and TrapsClaude Bagnis, Pascal Bailly, and Sylvie Chapel-Fernandes11 Use of a Macrophage-tropic GFP-tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral ReservoirsAmanda M. Brown 12 GFP-Lentiviral Vectors Targeting for NeuroAIDS Yuanan Lu13 Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene DeliveryAaron M. Jasnow, Donald G. Rainnie, Kimberly A. Maguschak, Jasmeer P. Chhatwal, and Kerry J. Ressler14 Selective Protein Expression Within the CNS Using Hybrid LentivirusFredrik Gussing, Ingrid van Marion, Igor Ralets, and Cecilia Lundberg 15 Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent ProteinBruce W. Banfield and Gregory A. Bird16 The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus ReplicationTravis J. Taylor and David M. Knipe17 Modified Bovine Herpesvirus 1 for Protein SecretionGünther M. Keil18 Identification of Viral Peptide Fragments for Vaccine DevelopmentZhanglin Lin, Shuang Li, and Yong Chen19 Use of GFP to Investigate Expression of Plant-derived VaccinesHong-Ye Li and Mee-Len Chye 20 Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP FusionsSophie Haupt, Angelika Ziegler, Graham Cowan, and Lesley Torrance21 Sendai Virus for Cancer ImmunotherapyYasuji Ueda, Mamoru Hasegawa, and Yoshikazu Yonemitsu22 Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic CellsQigui Yu, Ningjie Hu, and Mario Ostrowski23 Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity Misako Yoneda, Kentaro Fujita, Hiroki Sato, and Chieko Kai 24 Baculovirus-Mediated Gene Transfer into Mesenchymal Stem CellsChien-Tai Tsai, Ching-Kuang Chuang, and Yu-Chen Hu

Editorial Reviews

From the reviews:"One of the most useful modifications that have been made to viruses is the ability to encode tracers such as green fluorescent protein (GFP) . thereby allowing not only study of the virus itself, but also the behaviour of infected cells. As the title indicates, it is a methods text, giving useful practical details important to those attempting to carry out similar studies." (Christopher Ring, Microbiology Today, November, 2009)"This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized into 24 chapters dealing with the diverse viral delivery systems for green fluorescent proteins. . The book is written primarily for practicing scientists involved in the study of green fluorescent expression in live cells and experimental animals. . provides a number of updated protocols for the practicing investigator. . a valuable companion for all researchers involved in the use of green fluorescent proteins in biological systems." (Ruben Mestril, Doody's Review Service, September, 2009)"This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants . . The significance of viral applications of fluorescent proteins is well covered . . it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of life sciences." (Johannes Schmid, ChemBioChem, Vol. 10, 2009)"Cover the use of Green Fluorescent Protein (GFP) technology to study aspects of the replication of viruses and the majority of the chapters describe the use of viruses and GFP as research tools in a much wider context. . I enjoyed the book for the breadth of topics and snapshots of research . . a more likely home for this book is a university or institute library where its eclectic contents will have a sufficiently wide audience." (Paul Monaghan, Infocus Magazine, Issue 19, September, 2010)