Viral Vectors for Gene Therapy: Methods and Protocols by Otto-Wilhelm MertenViral Vectors for Gene Therapy: Methods and Protocols by Otto-Wilhelm Merten

Viral Vectors for Gene Therapy: Methods and Protocols

byOtto-Wilhelm MertenEditorMohamed Al-Rubeai

Hardcover | May 19, 2011

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The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular BiologyT series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.


Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.
Title:Viral Vectors for Gene Therapy: Methods and ProtocolsFormat:HardcoverDimensions:450 pagesPublished:May 19, 2011Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161779094X

ISBN - 13:9781617790942

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Table of Contents

1. Introduction to Viral Vectors

            James N. Warnock, Claire Daigre, and Mohamed Al-Rubeai


2. Introduction to Gene Therapy: A Clinical Aftermath

            Patrice P. Denèfle


3. Host Cells and Cell Banking

            Glyn N. Stacey and Otto-Wilhelm Merten


4. Overview of Current Scalable Methods for Purification of Viral Vectors

            María Mercedes Segura, Amine Kamen, and Alain Garnier


5. Methods to Construct Recombinant Adenovirus Vectors

            Miguel Chillon and Ramon Alemany


6. Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus

            Edwige Dormond and Amine A. Kamen


7. Manufacturing of Retroviruses

            Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana Amaral, Paula M. Alves, and Ana S. Coroadinha


8. Lentiviral Vectors

            Marc Giry-Laterrière, Els Verhoeyen, and Patrick Salmon


9. Adeno-Associated Viruses

            Mauro Mezzina and Otto-Wilhelm Merten


10. Manufacturing of Adeno-Associated Viruses, for Example: AAV2

            Haifeng Chen


11. Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses

            J. Fraser Wright and Olga Zelenaia


12. Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells

            Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. Mähönen, and Seppo Ylä-Herttuala


13. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors

            Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein


14. Manufacture of Measles Viruses

            Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, and Mark J. Federspiel


15. In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model

            Cecilia Frecha, Floriane Fusil, François-Loïc Cosset, and Els Verhoeyen


16. In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair)

            Kolja Gelse and Holm Schneider


17. Ethical Consideration

            Michael Fuchs


18. Clinical Trials of GMP Products in the Gene Therapy Field

            Kathleen B. Bamford

Editorial Reviews

From the reviews:"Intended for practitioners involved in the preclinical and clinical applications of gene therapy vectors. . students, scientists, and physicians specializing in gene therapy will find this book extremely useful. . Given the rapid advances in the field of gene therapy, this book is essential, providing useful information on the methodologies that involve virus production, transferring protocols, and evaluation of the efficacy of gene products and other important methodological concepts in gene therapy." (Omer Iqbal, Doody's Review Service, September, 2011)