Wound Healing: Methods and Protocols by Luisa A. DiPietroWound Healing: Methods and Protocols by Luisa A. DiPietro

Wound Healing: Methods and Protocols

EditorLuisa A. DiPietro, Aime L. Burns

Paperback | November 9, 2010

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Expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. These powerful models illustrate the biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure robust results.
Title:Wound Healing: Methods and ProtocolsFormat:PaperbackDimensions:484 pages, 9.02 × 5.98 × 0.07 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737296X

ISBN - 13:9781617372964

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Table of Contents

Part I. Experimental Models of Wound HealingA. In Vivo Animal ModelsExcisional Wound Healing: An Experimental ApproachStefan Frank and Heiko KämpferMethods in Reepithelialization: A Porcine Model of Partial-Thickness WoundsHeather N. Paddock, Gregory S. Schultz, and Bruce A. MastIncisional Wound Healing: Model and Analysis of Wound Breaking StrengthRichard L. Gamelli and Li-Ke HeAnimal Models of Ischemic Wound Healing: Toward an Approximation of Human Chronic Cutaneous Ulcers in Rabbit and RatMark Sisco and Thomas A. MustoeCorneal Injury: A Relatively Pure Model of Stromal-Epithelial Interactions in Wound HealingSteven E. Wilson, Rahul R. Mohan, Renato Ambrosio, and Rajiv R. MohanSubcutaneous Sponge ModelsDavid T. Efron and Adrian BarbulA Mouse Model of Burn Wounding and SepsisJulia M. Stevenson, Richard L. Gamelli, and Ravi ShankarA Porcine Burn ModelAdam J. Singer and Steve A. McClainWound Healing in Airways In VivoSteven R. WhiteMurine Models of Intestinal AnastomosesDavid L. Williams and I. William BrowderMurine Model of Peritoneal Adhesion FormationAndrew E. Jahoda, Mary Kay Olson, and Elizabeth J. KovacsMethods for Investigating Fetal Tissue RepairZiv M. Peled, Stephen M. Warren, Pierre J. Bouletreau, and Michael T. LongakerGrowth of Human Blood Vessels in Severe Combined Immunodeficient Mice: A New in Vivo Model System of AngiogenesisPeter J. Polverini, Jacques E. Nör, Martin C. Peters, and David J. MooneyB. Reviews of Specific Model SystemsTissue Repair in Models of Diabetes Mellitus: A ReviewDavid G. GreenhalghWound Healing Studies in Transgenic and Knockout Mice: A ReviewRichard Grose and Sabine WernerWound Repair in Aging: A ReviewMay J. Reed, Teruhiko Koike, and Pauli PuolakkainenC. Human Wound Healing ModelsSpecimen Collection and Analysis: Burn WoundsAreta Kowal-Vern and Barbara A. LatenserSuction Blister Model of Wound HealingVesa Koivukangas and Aarne OikarinenImplantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene ImplantsLars Nannestad Jorgensen, Søren Munk Madsen, and Finn GottrupD. In Vitro ModelsThe Fibroblast Populated Collagen Lattice: A Model of Fibroblast Collagen Interactions in RepairH. Paul EhrlichA Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell MotilityKirwin M. Providence, Lisa Staiano-Coico, and Paul J. HigginsHuman Skin Organ CultureIngrid MollIn Vitro Matrigel Angiogenesis ModelAnna M. Szpaderska and Luisa A. DiPietroPart II. Analysis and Manipulation of Wound HealingQuantification of Wound AngiogenesisQuentin E. H. Low and Luisa A. DiPietroIn Vivo Matrigel Migration and Angiogenesis AssaysKatherine M. MalindaEndothelial Cell Migration Assay: A Quantitative Assay for Prediction of In Vivo BiologyMark W. LingenAnalysis of Collagen SynthesisRobert F. DiegelmannMethod for Detection and Quantitation of Leukocytes During Wound HealingIulia Drugea and Aime L. BurnsDetection of Reactive Oxygen Intermediate Production by MacrophagesJorge E. Albina and Jonathan S. ReichnerMeasurement of Chemokines at the Protein Level in TissueRobert M. Strieter, Marie D. Burdick, John A. Belperio, and Michael P. KeaneMethods of Measuring Oxygen in WoundsHarriet W. Hopf, Thomas K. Hunt, Heinz Scheuenstuhl, Judith M. West, Lisa M. Humphrey, and Mark D. Rollins.Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle CellsMartin Graham and Amy WilleyUse of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound RepairNicole S. Gibran and F. Frank IsikParticle-Mediated Gene Therapy of WoundsJeffrey M. Davidson, Sabine A. Eming, and Jayasri DasguptaIndex

Editorial Reviews

From the reviews:"Clinical and basic science researchers with an interest in wound healing of various kinds are an appropriate audience for this work. Editors and authors represent some of the leading wound healing laboratories in the United States with a smaller number of presentations from overseas. . .Chapters are written in a straightforward manner. . .This book is a collection of techniques and insights which belongs on the shelf of laboratories or research organizations having an interest in wounds." -Doody's Health Sciences Book Review Journal"This informative volume provides a highly practical collection of widely used model systems and methods for studying the injury repair process and is therefore highly recommended as a valuable reference source for all individuals with interests in wound healing." - Carbohydrate Polymers"The editors have done an impressive job of editing the book into both a practical and readable style. It is highly recommended for any group that wishes to participate in this exciting and expanding field of research." -British Journal of Surgery"This book . provides a range of classical and more recent tools in wound research. . an impressive collection of both animal and in vitro-based techniques useful for experimental studies of tissue damage and repair. . Each chapter has a concluding notes section offering valuable suggestions and warnings based on practical experience. The book is highly recommended for all basic and clinical researchers involved in tissue repair biology and particularly those with an interest in experimental wound modelling and analysis." (Ola Rollman, Acta Dermato-Venereologica, Vol. 85, 2005)