Wound Healing: Methods and Protocols by Luisa A. DiPietroWound Healing: Methods and Protocols by Luisa A. DiPietro

Wound Healing: Methods and Protocols

byLuisa A. DiPietroEditorAime L. Burns

Paperback | November 9, 2010

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During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing. The combination of new and classic approaches has allowed scientists to make exciting discoveries in the field of tissue repair, resulting in an explosion of information about the healing p- cess. Importantly, these new findings have great relevance beyond wound healing itself. The injury repair process cuts across many disciplines, exte- ing to such broad fields as cancer, inflammation, and atherosclerosis. The relevance of the field to these many disciplines has generated great interest in models and methods for the study of wound healing. The goal of Wound Healing: Methods and Protocols is to provide scientists from many dis- plines with a compendium of classic and contemporary protocols from r- ognized experts in the field of wound healing. We hope this volume will be useful not only to those working within the field itself, but also to scientists from other disciplines who wish to adapt wound healing models to their own experimental needs. The process of wound healing encompasses many different biologic p- cesses, including epithelial growth and differentiation, fibrous tissue prod- tion and function, angiogenesis, and inflammation.
Title:Wound Healing: Methods and ProtocolsFormat:PaperbackDimensions:468 pages, 22.9 × 15.2 × 0.17 inPublished:November 9, 2010Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737296X

ISBN - 13:9781617372964

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Table of Contents

Part I. Experimental Models of Wound Healing A. In Vivo Animal Models Excisional Wound Healing: An Experimental Approach Stefan Frank and Heiko Kämpfer Methods in Reepithelialization: A Porcine Model of Partial-Thickness Wounds Heather N. Paddock, Gregory S. Schultz, and Bruce A. Mast Incisional Wound Healing: Model and Analysis of Wound Breaking Strength Richard L. Gamelli and Li-Ke He Animal Models of Ischemic Wound Healing: Toward an Approximation of Human Chronic Cutaneous Ulcers in Rabbit and Rat Mark Sisco and Thomas A. Mustoe Corneal Injury: A Relatively Pure Model of Stromal-Epithelial Interactions in Wound Healing Steven E. Wilson, Rahul R. Mohan, Renato Ambrosio, and Rajiv R. Mohan Subcutaneous Sponge Models David T. Efron and Adrian Barbul A Mouse Model of Burn Wounding and Sepsis Julia M. Stevenson, Richard L. Gamelli, and Ravi Shankar A Porcine Burn Model Adam J. Singer and Steve A. McClain Wound Healing in Airways In Vivo Steven R. White Murine Models of Intestinal Anastomoses David L. Williams and I. William Browder Murine Model of Peritoneal Adhesion Formation Andrew E. Jahoda, Mary Kay Olson, and Elizabeth J. Kovacs Methods for Investigating Fetal Tissue Repair Ziv M. Peled, Stephen M. Warren, Pierre J. Bouletreau, and Michael T. Longaker Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice: A New in Vivo Model System of Angiogenesis Peter J. Polverini, Jacques E. Nör, Martin C. Peters, and David J. Mooney B. Reviews of Specific Model Systems Tissue Repair in Models of Diabetes Mellitus: A Review David G. Greenhalgh Wound Healing Studies in Transgenic and Knockout Mice: A Review Richard Grose and Sabine Werner Wound Repair in Aging: A Review May J. Reed, TeruhikoKoike, and Pauli Puolakkainen C. Human Wound Healing Models Specimen Collection and Analysis: Burn Wounds Areta Kowal-Vern and Barbara A. Latenser Suction Blister Model of Wound Healing Vesa Koivukangas and Aarne Oikarinen Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants Lars Nannestad Jorgensen, Soren Munk Madsen, and Finn Gottrup D. In Vitro Models The Fibroblast Populated Collagen Lattice: A Model of Fibroblast Collagen Interactions in Repair H. Paul Ehrlich A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility Kirwin M. Providence, Lisa Staiano-Coico, and Paul J. Higgins Human Skin Organ Culture Ingrid Moll In Vitro Matrigel Angiogenesis Model Anna M. Szpaderska and Luisa A. DiPietro Part II. Analysis and Manipulation of Wound Healing Quantification of Wound Angiogenesis Quentin E. H. Low and Luisa A. DiPietro In Vivo Matrigel Migration and Angiogenesis Assays Katherine M. Malinda Endothelial Cell Migration Assay: A Quantitative Assay for Prediction of In Vivo Biology Mark W. Lingen Analysis of Collagen Synthesis Robert F. Diegelmann Method for Detection and Quantitation of Leukocytes During Wound Healing Iulia Drugea and Aime L. Burns Detection of Reactive Oxygen Intermediate Production by Macrophages Jorge E. Albina and Jonathan S. Reichner Measurement of Chemokines at the Protein Level in Tissue Robert M. Strieter, Marie D. Burdick, John A. Belperio, and Michael P. Keane Methods of Measuring Oxygen in Wounds Harriet W. Hopf, Thomas K. Hunt, Heinz Scheuenstuhl, Judith M. West, Lisa M. Humphrey, and Mark D. Rollins. Isolation, Culture, and

Editorial Reviews

From the reviews:"Clinical and basic science researchers with an interest in wound healing of various kinds are an appropriate audience for this work. Editors and authors represent some of the leading wound healing laboratories in the United States with a smaller number of presentations from overseas. . .Chapters are written in a straightforward manner. . .This book is a collection of techniques and insights which belongs on the shelf of laboratories or research organizations having an interest in wounds." -Doody's Health Sciences Book Review Journal"This informative volume provides a highly practical collection of widely used model systems and methods for studying the injury repair process and is therefore highly recommended as a valuable reference source for all individuals with interests in wound healing." - Carbohydrate Polymers"The editors have done an impressive job of editing the book into both a practical and readable style. It is highly recommended for any group that wishes to participate in this exciting and expanding field of research." -British Journal of Surgery"This book . provides a range of classical and more recent tools in wound research. . an impressive collection of both animal and in vitro-based techniques useful for experimental studies of tissue damage and repair. . Each chapter has a concluding notes section offering valuable suggestions and warnings based on practical experience. The book is highly recommended for all basic and clinical researchers involved in tissue repair biology and particularly those with an interest in experimental wound modelling and analysis." (Ola Rollman, Acta Dermato-Venereologica, Vol. 85, 2005)