Enzymology by T. DevasenaEnzymology by T. Devasena


byT. Devasena

Paperback | December 11, 2010

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Enzymology is designed as a full-fledge textbook for the undergraduate engineering students of Biotechnology and Chemical Engineering. In addition, this book would also serve as an invaluable reference for students who are pursuing their graduate and postgraduate degree programs inBiotechnology, and all other life sciences programs that offer a course on Enzymes.The book covers all the fundamental and inevitable concepts like Enzyme Kinetics, Enzyme Inhibition, Enzyme Activity Regulation and proceeds in to discussion of applications of enzymes in various domains including Molecular Biology, Cloning and Genetic Engineering. A separate chapter has beendevoted to the study of Enzyme Engineering and Technology, which the engineering students would find useful.Comprehensive in its coverage of topics, the book is rich in features like illustrations supporting the theoretical discussion, chapter-end summary, glossary of important terms and review questions to reinforce the learning. Numerical problems too have been provided in all the relevantchapters.
Dr T. Devasena is a faculty, Department of Biotechnology, Mother Teresa Women's University, Kodaikanal. She has around 8 years of post-doctoral experience in teaching and research, which includes a brief stint as a Research Scientist in a project at the Centre for Micronutrient Research (CMNR), Annamalai University in association with...
Title:EnzymologyFormat:PaperbackDimensions:568 pages, 9.84 × 7.48 × 0.03 inPublished:December 11, 2010Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0198064438

ISBN - 13:9780198064435


Table of Contents

1. Introduction to Enzymology1.1. Biocatalysts1.2. Structure of enzymes1.3. Models for monosubstrate reaction1.4. Models for bisubstrate reaction1.5. Monomeric and oligomeric enzymes1.6. Multisubstrate reaction1.7. Enzyme localization - Histoenzymology1.8. Enzyme assay1.9. Purification of enzymes from natural resource1.10. Fractionation and characterization of proteins (Enzymes)Case studyChapter summaryTerms to rememberStudy questionsSolved problemsExercise problems2. Mechanism of Enzyme Action2.1. Mechanistic enzymology and intermediary enzymology2.2. Strategies of enzyme action2.3. Advantages of mechanistic enzymology2.4. Multienzyme complex2.5. Mechanism of action of some important EnzymesCase studyChapter summaryTerms to rememberStudy questions3. Enzyme Kinetics3.1. Definition3.2. Order of a reaction3.3. The substrate velocity Plot3.4. Michaelis Menten Reaction3.5. MM rate equation3.6. Two constants in enzyme catalyzed reaction3.7. Transformation of MM plot into linear plot3.8. Bisubstrate reaction3.9. Haldane equationChapter summaryTerms to rememberStudy questionsSolved ProblemsExercise Problems4. Enzyme Inhibition4.1. Enzyme Inhibitors4.2. Types of Enzyme inhibition4.3. Active site mapping (ASM)Case studyChapter summaryTerms to rememberStudy questions5. Regulation of Enzyme Activity5.1. Need for enzyme regulation5.2. Methods of enzyme regulationCase studyChapter summaryTerms to rememberStudy questions6. Coenzymes6.1. Coenzymes and cosubstrates6.2. Function6.3. Types of coenzymes6.4 Other Coenzymes6.5. cofactorsChapter summaryTerms to rememberStudy questions7. Usiztnes, Abzymes, Ribozymes, and Deoxyribozymes7.1 Isozymes7.2.Abzymes7.3. Ribozymes7.4.Deoxyribozyme (DNAzyme)Chapter summaryTerms to rememberStudy questions8. Applications of Enzymes8.1. Enzymes as diagnostic agents8.2. Enzymes as analytical agents8.3. Enzymes as therapeutic agents8.4. Industrial applications8.5. Enzymes of Biological importance8.6. Enzyme business in India and abroadCase studyChapter summaryTerms to rememberStudy questions9. Enzymes in Molecular Biology, Cloning and Genetic Engineering9.1. Enzymes in replication9.2. Enzymes in transcription9.3. Enzymes in translation9.4. Nucleases9.5. Ligase9.6. Alkaline phosphatase (ALP) and Polynucleotide kinase9.7. Proteinase K9.8. Terminal deoxynucleotidyl transferase9.9. Reverse transcriptase9.10. Telomere and telomerase9.11. Reporter enzymes9.12. Other enzymesCase studyChapter summaryTerms to rememberStudy questions10. Chapter Outline10.1. Definition10.2. Subtilisin- The most common enzyme used in mutation studies and protein engineering.10.3. Types of mutation10.4. Selection of mutants10.5. Common mutations in target amino acid residues of enzymes10.6. Role of mutation in understanding the catalytic process- tyrosl-tRNA synthetase as an example10.7. Enzyme mutations in diseased stateCase studySummaryTerms to rememberStudy questions11. Synzymes11.1. Definition for synzymes11.2. Criteria for creating synzymes11.3. Flavopapain - A typical example for synzyme11.4. Some important synzymes11.5. Antioxidant Synzymes in Blood substituteChapter summaryTerms to rememberStudy questions12. Enzyme Models- Artizymes12.1. Definition12.2. Synonyms for enzyme models12.3. Biomimetics12.4. Features of enzyme model12.5. Advantages of enzyme model12.6.Tailoring enzyme models12.7. Demerits of artizymology12.8. Examples for engineered artizymes12.9. Artificial Metallozymes12.10. Application of artizyme- to understand the catalytic efficiency12.10.1. Understanding the catalytic efficiency of cytochrome oxidaseCase studySummaryTerms to remember13. Immobilized Enzymes13.1. Definition13.2. Advantages of immobilization13.3. Methods of immobilizationChapter summaryTerms to rememberStudy questions14. Enzyme Technology and Enzyme Engineering14.1. Production of enzymes of interest14.2. Enzyme reactor14.3. Biosensors and Biochips14.4. Enzyme electrode14.5. Inhibited enzyme electrode14.6. ELISA (Enzyme linked immunosorbent Assay)14.7. Engineered Enzymes14.8. Enzymes and bioinformaticsCase studyChapter summaryTerms to rememberStudy questions