Molecular Cytogenetics: Protocols and Applications by Yao-Shan FanMolecular Cytogenetics: Protocols and Applications by Yao-Shan Fan

Molecular Cytogenetics: Protocols and Applications

EditorYao-Shan Fan

Paperback | November 10, 2010

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Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.
Title:Molecular Cytogenetics: Protocols and ApplicationsFormat:PaperbackDimensions:426 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373001

ISBN - 13:9781617373008

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Table of Contents

Part I. Basic Concepts and TechniquesMolecular Cytogenetics in Medicine: An OverviewYao-Shan FanLabeling Fluorescence In Situ Hybridization Probes for Genomic TargetsLarry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, and Kim A. WilberLabeling Fluorescence In Situ Hybridization Probes for RNA TargetsRamesh Ramakrishnan and Larry E. MorrisonBasic FISH Techniques and TroubleshootingSue Van Stedum and Walter KingPart II. Evolving Techniques and ApplicationsChromosome MicrodissectionXin-Yuan GuanPrimed In Situ LabelingJørn KochSpectral KaryotypingJane Bayani and Jeremy A. SquireMulticolor FISHSyed M. Jalal and Mark E. LawCharacterization of Constitutional Chromosome Abnormalities by Comparative Genomic HybridizationBrynn Levy and Kurt HirschhornColor BandingVicki L. Hopwood, Christi J. Hada, and Armand B. GlassmanMulticolor Fiber FISHJaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap, Philip M. Kluin, and Ed SchuuringMulti-Telomere FISHSamantha J. L. Knight and Jonathan FlintFluorescence Genotyping for Screening Cryptic Telomeric RearrangementsLaurence Colleaux, Solange Heuertz, Florence Molinari, and Marlene RioMicroarray CGH, Ben Beheshti, Paul C. Park, Ilan Braude, and Jeremy A. SquirePart III. Special Applications in Chromosomal DisordersMolecular Approaches for Delineating Marker ChromosomesMichael D. Graf and Stuart SchwartzInterphase FISH for Prenatal Diagnosis of Common AneuploidiesBaruch Feldman, Ayala Aviram-Goldring, and Mark I. EvansPrenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood, Farideh Z. Bischoff, Dianne X. Dang, Dorothy E. Lewis, and Joe Leigh SimpsonPreimplantation FISH Diagnosis of AneuploidiesYury Verlinsky, Jeanine Cieslak, and Anver KulievMicrodeletion Syndromes: Characteristics and DiagnosisStuart Schwartz and Michael D. GrafMolecular Detection of Uniparental DisomyWendy P. RobinsonMolecular Cytogenetics in Reproductive PathologyHélène Bruyère, Evica Rajcan-Separovic, and Dagmar K. KalousekPart IV. Special Applications in OncologyInterphase FISH Studies of Chronic Myeloid Leukemia, Gordon W. DewaldChromogenic In Situ Hybridization and FISH in PathologyBae-Li Hsi, Sheng Xiao, and Jonathan A. FletcherFISH Detection of HER2 Amplification in Breast CancerRosemary E. Mueller and Frances P. O'MalleyComparative Genomic Hybridization in Cancer InvestigationsMarjan M. Weiss, Mario A. J. A. Hermsen, Gerrit A. Meijer, and Paul J. van DiestSimultaneous Fluorescence Immunophenotyping and FISH on Tumor CellsYanming Zhang and Brigitte SchlegelbergerBAC Resource for Molecular CytogeneticsXiao-Ning Chen and Julie R. KorenbergIndex

Editorial Reviews

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent."Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics