Epstein-Barr Virus Protocols by Joanna B. WilsonEpstein-Barr Virus Protocols by Joanna B. Wilson

Epstein-Barr Virus Protocols

EditorJoanna B. Wilson, Gerhard H. W. May

Paperback | November 10, 2010

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Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer.
Title:Epstein-Barr Virus ProtocolsFormat:PaperbackDimensions:452 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371378

ISBN - 13:9781617371370

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Table of Contents

I. Genome and Transcript AnalysesEpstein-Barr Virus: The B95-8 Strain MapPaul J. FarrellAnalysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCRAnn L. KirchmaierGenetic Analysis and Gene Expression with Mini-Epstein-Barr Virus PlasmidsEllen Kilger and Wolfgang HammerschmidtConstruction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs)Paul R. Smith. Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous CellsKenneth G. Laing, Volker Matys, and Michael J. ClemensVisualizing EBV Expression Patterns by FISHAnna SzelesII. Viral DetectionIn Situ Detection of Epstein-Barr Virus DNA and Viral Gene ProductsGerald Niedobitek and Hermann HerbstPhenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue SectionsHermann Herbst and Gerald NiedobitekDetection of EBV Infection at the Single-Cell Level: Precise Quantitation of Virus-Infected Cells In VivoGregory J. Babcock, Emily M. Miyashita-Lin, and David A. Thorley-LawsonDetection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In VivoLisa L. Decker, Gregory J. Babcock, and David A. Thorley-LawsonIII. Culture MethodsVirus IsolationLindsey M. Hutt-Fletcher and Susan M. TurkGeneration of Lymphoblastoid Cell Lines (LCLs)Teresa Frisan, Victor Levitsky, and Maria MasucciCell Cycle Distribution of B-Lymphocytes and Cell LinesAlison J. SinclairIntroduction of Plasmid Vectors into Cells Via ElectroporationAnn L. KirchmaierMalignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 GeneTadamasa OokaTransient Gene Expression and MACS EnrichmentDieter Kube and Martina VockerodtIn Vitro Assays to Study Epithelial Cell GrowthChristopher W. Dawson and Lawrence S. YoungIn Vitro Assays to Study Epithelial Cell DifferentiationChristopher W. Dawson, and Lawrence S. YoungCell Sensitivity Assays: Quantitative Detection of Apoptotic Cells In Vitro Using the TUNEL AssayNeil A. Jones and Caroline DiveQualitative Detection of Apoptotic Cells Assessed by DNA FragmentationAmanda J. McIlwrathIV. Immune AssaysRegression AssayTeresa Frisan, Victor Levitsky, and Maria MasucciGeneration of Polyclonal EBV-Specific CTL Cultures and ClonesVictor Levitsky, Teresa Frisan, and Maria MasucciDetermination of Antigen and Fine Peptide Specificity of EBV-Specific CTLsVictor Levitsky, Teresa Frisan, and Maria MasucciLimiting Dilution AssayTeresa Frisan, Victor Levitsky, and Maria MasucciV. Viral Protein DetectionAntibodies for Detecting EBV Latent ProteinsMartin Rowe, and Anja MehlDetection of EBV Latent Proteins by Western BlottingMartin Rowe and Matthew JonesBiosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic AnalysisLindsey M. Hutt-Fletcher and Susan M. TurkVI. Protein-Protein, Protein-DNA, and Protein-RNA InteractionsThe Yeast Two-Hybrid Assay to Identify Interacting ProteinsKenneth M. IzumiIdentification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion ProteinsJames J.-D. Hsieh and S. Diane HaywardMagnetic DNA Affinity Purification of a Cellular Transcription FactorLothar J. Strobl and Ursula Zimber-StroblScreening an Expression Library with a DNA Binding SiteQin Zhang and Eduardo A. MontalvoAnalysis of DNA Binding Proteins by Mobility Shift AssayYi-Chun James Wang and Eduardo A. MontalvoAnalysis of RNA-Protein Interactions of the EBV-Encoded Small RNAs, the EBERs: In Vitro AssaysKenneth G. Laing, Androulla Elia, Ian W. Jeffrey, and Michael J. ClemensVII. Protein Activity AssaysChimeric and Mutated Variants of LMP1: A Helpful Tool to Analyze the Structure-Function Relationship of a PseudoreceptorOlivier Gires, Marius Ueffing, and Wolfgang HammerschmidtAssaying the Activity of Kinases Regulated by LMP1Arnd KieserIn Vitro Assays for the Detection of Protein Tyrosine Phosphorylation and Protein Tyrosine Kinase ActivitiesSara Fruehling and Richard LongneckerVIII. Tissue and In Vivo ProtocolsAnalysis of Apoptosis in Tissue SectionsVicki Save, Philip J. Coates, and Peter A. HallConsiderations in Generating Transgenic Mice: DNA, RNA, and Protein Extractions from Tissues-Rapid and Effective BlottingJoanna B. Wilson and Mark E. DrotarIn Vivo Assay of Cellular ProliferationJohn CurranTopical Chemical Carcinogen Treatment in MiceJohn CurranSeparation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte CultureJennifer Macdiarmid and Joanna B. WilsonSelection and Enrichment of B Cells from Lymphoid TissuesPenelope Tsimbouri, Marie Anne O'Donnell, and Joanna B. WilsonDetection of Immunoglobulin Gene RearrangementsRobert G. Caldwell and Richard Longnecker

Editorial Reviews

"Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer." - Anticancer Research"Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter." -Today's Life Science"This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive..." "Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology." -Microbiology Today"Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design." -Tumori"This book is highly recommended for collections serving researchers in the area of molecular biology." -E-Streams